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  PPFIA1 drives active alpha 5 beta 1 integrin recycling and controls fibronectin fibrillogenesis and vascular morphogenesis

Mana, G., Clapero, F., Panieri, E., Panero, V., Böttcher, R. T., Tseng, H.-Y., et al. (2016). PPFIA1 drives active alpha 5 beta 1 integrin recycling and controls fibronectin fibrillogenesis and vascular morphogenesis. Nature Communications, 7: 13546. doi:10.1038/ncomms13546.

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 Creators:
Mana, Giulia1, Author
Clapero, Fabiana1, Author
Panieri, Emiliano1, Author
Panero, Valentina1, Author
Böttcher, Ralph T.2, Author           
Tseng, Hui-Yuan2, Author           
Saltarin, Federico1, Author
Astanina, Elena1, Author
Wolanska, Katarzyna I.1, Author
Morgan, Mark R.1, Author
Humphries, Martin J.1, Author
Santoro, Massimo M.1, Author
Serini, Guido1, Author
Valdembri, Donatella1, Author
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1external, ou_persistent22              
2Fässler, Reinhard / Molecular Medicine, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565147              

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Free keywords: PROTEIN-TYROSINE-PHOSPHATASE; TRANS-GOLGI NETWORK; CELL-MIGRATION; MYOCARDIAL MIGRATION; MOLECULAR-MECHANISMS; ENDOTHELIAL-CELLS; MATRIX FORMATION; LUMEN FORMATION; EARLY ENDOSOMES; SYNTAXIN 6Science & Technology - Other Topics;
 Abstract: Basolateral polymerization of cellular fibronectin (FN) into a meshwork drives endothelial cell (EC) polarity and vascular remodelling. However, mechanisms coordinating alpha 5 beta 1 integrin-mediated extracellular FN endocytosis and exocytosis of newly synthesized FN remain elusive. Here we show that, on Rab21-elicited internalization, FN-bound/active alpha 5 beta 1 is recycled to the EC surface. We identify a pathway, comprising the regulators of post-Golgi carrier formation PI4KB and AP-1A, the small GTPase Rab11B, the surface tyrosine phosphatase receptor PTPRF and its adaptor PPFIA1, which we propose acts as a funnel combining FN secretion and recycling of active alpha 5 beta 1 integrin from the trans-Golgi network (TGN) to the EC surface, thus allowing FN fibrillogenesis. In this framework, PPFIA1 interacts with active alpha 5 beta 1 integrin and localizes close to EC adhesions where post-Golgi carriers are targeted. We show that PPFIA1 is required for FN polymerization-dependent vascular morphogenesis, both in vitro and in the developing zebrafish embryo.

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Language(s): eng - English
 Dates: 2016-11-23
 Publication Status: Published online
 Pages: 20
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000388323000001
DOI: 10.1038/ncomms13546
 Degree: -

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Title: Nature Communications
  Abbreviation : Nat. Commun.
Source Genre: Journal
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Publ. Info: London : Nature Publishing Group
Pages: - Volume / Issue: 7 Sequence Number: 13546 Start / End Page: - Identifier: ISSN: 2041-1723
CoNE: https://pure.mpg.de/cone/journals/resource/2041-1723