非表示:
キーワード:
PROTEIN-TYROSINE-PHOSPHATASE; TRANS-GOLGI NETWORK; CELL-MIGRATION;
MYOCARDIAL MIGRATION; MOLECULAR-MECHANISMS; ENDOTHELIAL-CELLS; MATRIX
FORMATION; LUMEN FORMATION; EARLY ENDOSOMES; SYNTAXIN 6Science & Technology - Other Topics;
要旨:
Basolateral polymerization of cellular fibronectin (FN) into a meshwork drives endothelial cell (EC) polarity and vascular remodelling. However, mechanisms coordinating alpha 5 beta 1 integrin-mediated extracellular FN endocytosis and exocytosis of newly synthesized FN remain elusive. Here we show that, on Rab21-elicited internalization, FN-bound/active alpha 5 beta 1 is recycled to the EC surface. We identify a pathway, comprising the regulators of post-Golgi carrier formation PI4KB and AP-1A, the small GTPase Rab11B, the surface tyrosine phosphatase receptor PTPRF and its adaptor PPFIA1, which we propose acts as a funnel combining FN secretion and recycling of active alpha 5 beta 1 integrin from the trans-Golgi network (TGN) to the EC surface, thus allowing FN fibrillogenesis. In this framework, PPFIA1 interacts with active alpha 5 beta 1 integrin and localizes close to EC adhesions where post-Golgi carriers are targeted. We show that PPFIA1 is required for FN polymerization-dependent vascular morphogenesis, both in vitro and in the developing zebrafish embryo.
