English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Type 3 Fimbriae Encoded on Plasmids Are Expressed from a Unique Promoter without Affecting Host Motility, Facilitating an Exceptional Phenotype That Enhances Conjugal Plasmid Transfer

Madsen, J. S., Riber, L., Kot, W., Basfeld, A., Burmolle, M., Hansen, L. H., et al. (2016). Type 3 Fimbriae Encoded on Plasmids Are Expressed from a Unique Promoter without Affecting Host Motility, Facilitating an Exceptional Phenotype That Enhances Conjugal Plasmid Transfer. PLoS One, 11(9): e0162390. doi:10.1371/journal.pone.0162390.

Item is

Files

show Files
hide Files
:
journal.pone.0162390.PDF (Publisher version), 2MB
Name:
journal.pone.0162390.PDF
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
© 2016 Madsen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License.
License:
-
:
3831351.zip (Supplementary material), 5MB
Name:
3831351.zip
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/zip / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Madsen, Jonas Stenlokke1, Author
Riber, Leise1, Author
Kot, Witold1, Author
Basfeld, Alrun2, Author              
Burmolle, Mette1, Author
Hansen, Lars Hestbjerg1, Author
Sorensen, Soren Johannes1, Author
Affiliations:
1external, ou_persistent22              
2Gruber, Stephan / Chromosome Organization and Dynamics, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565151              

Content

show
hide
Free keywords: ESCHERICHIA-COLI K-12; CYCLIC DI-GMP; BIOFILM FORMATION; KLEBSIELLA-PNEUMONIAE; GENE-EXPRESSION; H-NS; MOLECULAR CHARACTERIZATION; ANTIMICROBIAL RESISTANCE; PSEUDOMONAS-AERUGINOSA; NUCLEOTIDE-SEQUENCEScience & Technology - Other Topics;
 Abstract: Horizontal gene transfer (HGT), the transmission of genetic material to a recipient that is not the progeny of the donor, is fundamental in bacterial evolution. HGT is often mediated by mobile genetic elements such as conjugative plasmids, which may be in conflict with the chromosomal elements of the genome because they are independent replicons that may petition their own evolutionary strategy. Here we study differences between type 3 fimbriae encoded on wild type plasmids and in chromosomes. Using known and newly characterized plasmids we show that the expression of type 3 fimbriae encoded on plasmids is systematically different, as MrkH, a c-di-GMP dependent transcriptional activator is not needed for strong expression of the fimbriae. MrkH is required for expression of type 3 fimbriae of the Klebsiella pneumoniae chromosome, where from the fimbriae operon (mrkABCDF) of plasmids is believed to have originated. We find that mrkABCDFs of plasmids are highly expressed via a unique promoter that differs from the original Klebsiella promoter resulting in fundamental behavioral consequences. Plasmid associated mrkABCDFs did not influence the swimming behavior of the host, that hereby acquired an exceptional phenotype being able to both actively swim (planktonic behavior) and express biofilm associated fimbriae (sessile behavior). We show that this exceptional phenotype enhances the conjugal transfer of the plasmid.

Details

show
hide
Language(s): eng - English
 Dates: 2016-09-14
 Publication Status: Published online
 Pages: 19
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: PLoS One
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: San Francisco, CA : Public Library of Science
Pages: - Volume / Issue: 11 (9) Sequence Number: e0162390 Start / End Page: - Identifier: ISSN: 1932-6203
CoNE: https://pure.mpg.de/cone/journals/resource/1000000000277850