English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  The ras-related mouse ypt1 protein can functionally replace the YPT1 gene product in yeast.

Haubruck, H., Prange, R., Vorgias, C., & Gallwitz, D. (1989). The ras-related mouse ypt1 protein can functionally replace the YPT1 gene product in yeast. EMBO Journal, 8(5), 1427-1432.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-4958-5 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-495C-E
Genre: Journal Article

Files

show Files
hide Files
:
2384811.pdf (Publisher version), 2MB
Name:
2384811.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Haubruck, H.1, Author              
Prange, R., Author
Vorgias, C., Author
Gallwitz, D.1, Author              
Affiliations:
1Department of Molecular Genetics, MPI for biophysical chemistry, Max Planck Society, ou_578622              

Content

show
hide
Free keywords: -
 Abstract: The protein-coding region of the essential Saccharomyces cerevisiae YPT1 gene coding for a ras-related, guanine-nucleotide-binding protein was exchanged in chromosome VI by the protein-coding segment of either the mouse ypt1 gene or the v-Ki-ras gene, and different chimeric YPT1-v-Ki-ras genes. The mouse ypt1 protein with 71% of identical residues compared with the yeast Ypt1 protein could functionally fully replace its yeast homologue as long as the mouse gene was overexpressed under transcriptional control of the inducible GAL10 promoter. In contrast, neither the viral Ki-ras nor the hybrid proteins were able to substitute for the loss of YPT1 gene function. This study suggests that different parts of the yeast Ypt1 protein are required for the interaction with cellular targets and that these essential parts are conserved in the mammalian ypt1 protein.

Details

show
hide
Language(s): eng - English
 Dates: 1989-05
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: -
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: EMBO Journal
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 8 (5) Sequence Number: - Start / End Page: 1427 - 1432 Identifier: -