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  Characterization, by size, density, osmotic fragility, and immunoaffinity, of acetylcholine- and vasoactive intestinal polypeptide-containing storage particles from myenteric neurones of the guinea-pig.

Agoston, D., & Whittaker, V. P. (1989). Characterization, by size, density, osmotic fragility, and immunoaffinity, of acetylcholine- and vasoactive intestinal polypeptide-containing storage particles from myenteric neurones of the guinea-pig. Journal of Neurochemistry, 52(5), 1474-1480. doi:10.1111/j.1471-4159.1989.tb09196.x.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-4965-7 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-496E-6
Genre: Journal Article

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Agoston, D.1, Author              
Whittaker, V. P.1, Author              
Affiliations:
1Abteilung Neurochemie, MPI for biophysical chemistry, Max Planck Society, ou_578555              

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 Abstract: Abstract: When cytoplasmic extracts of guinea-pig myenteric neurones are submitted to centrifugal density gradient fractionation in a zonal rotor acetylcholine is bimodally distributed in the gradient, in a peak (I) rich in synaptic vesicles of the classic type and in a denser peak (II/VI) rich in densecored vesicles and vasoactive intestinal polypeptide (VIP). The putative stable synaptic vesicle markers synaptophysin (p38), vesicular proteoglycan, and Mg2+-activated ATPase were also bimodally distributed, with a peak coincident with peak I and another, broader peak embracing peak II/VI, and neighbouring peaks of other neuropeptides resolved from peak II/VI by the density gradient separation procedure used. To establish whether the stable markers, acetylcholine and VIP in peak II/VI were present in one particle or several, attempts were made to separate them by particle-exclusion chromatography and differential osmotic fragility. These were unsuccessful, leading us to conclude that the storage particles in peak II/VI contain both neurotransmitters and all three putative stable synaptic vesicle markers. It is suggested that such particles are the counterparts, in cholinergic neurones of the myenteric plexus, of the dense-cored, enkephalin- and noradrenaline-containing vesicles of certain adrenergic neurones and, like the latter, may exist in a precursor–product relationship with the classic synaptic vesicles containing the small-molecular-mass transmitters and found in the same nerve terminals.

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Language(s): eng - English
 Dates: 1989-05
 Publication Status: Published in print
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 Rev. Method: Peer
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Title: Journal of Neurochemistry
Source Genre: Journal
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Pages: - Volume / Issue: 52 (5) Sequence Number: - Start / End Page: 1474 - 1480 Identifier: -