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  Engineering extrinsic disorder to control protein activity in living cells

Dagliyan, O., Tarnawski, M., Chu, P.-H., Shirvanyants, D., Schlichting, I., Dokholyan, N. V., et al. (2016). Engineering extrinsic disorder to control protein activity in living cells. Science, 354(6318), 1441-1444. doi:10.1126/science.aah3404.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-4A90-0 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-4A91-E
Genre: Journal Article

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Science_354_2016_1441.pdf (Any fulltext), 2MB
 
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 Creators:
Dagliyan, Onur, Author
Tarnawski, Miroslaw1, Author              
Chu, Pei-Hsuan, Author
Shirvanyants, David, Author
Schlichting, Ilme1, Author              
Dokholyan, Nikolay V., Author
Hahn, Klaus M., Author
Affiliations:
1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              

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 Abstract: Optogenetic and chemogenetic control of proteins has revealed otherwise inaccessible facets of signaling dynamics. Here, we use light- or ligand-sensitive domains to modulate the structural disorder of diverse proteins, thereby generating robust allosteric switches. Sensory domains were inserted into nonconserved, surface-exposed loops that were tight and identified computationally as allosterically coupled to active sites. Allosteric switches introduced into motility signaling proteins (kinases, guanosine triphosphatases, and guanine exchange factors) controlled conversion between conformations closely resembling natural active and inactive states, as well as modulated the morphodynamics of living cells. Our results illustrate a broadly applicable approach to design physiological protein switches.

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Language(s): eng - English
 Dates: 2016-06-122016-11-162016-11-16
 Publication Status: Published in print
 Pages: 4
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
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Title: Science
Source Genre: Journal
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Publ. Info: Washington, D.C. : American Association for the Advancement of Science
Pages: - Volume / Issue: 354 (6318) Sequence Number: - Start / End Page: 1441 - 1444 Identifier: ISSN: 0036-8075
CoNE: https://pure.mpg.de/cone/journals/resource/991042748276600_1