English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Unlocking cancer glycomes from histopathological formalin-fixed and paraffin-embedded (FFPE) tissue microdissections

Hinneburg, H., Korać, P., Schirmeister, F., Gasparov, S., Seeberger, P. H., Zoldoš, V., et al. (2017). Unlocking cancer glycomes from histopathological formalin-fixed and paraffin-embedded (FFPE) tissue microdissections. Molecular and Cellular Proteomics, 16(4), 524-536. doi:10.1074/mcp.M116.062414.

Item is

Files

show Files
hide Files
:
Article.pdf (Publisher version), 4MB
 
File Permalink:
-
Name:
Article.pdf
Description:
-
OA-Status:
Visibility:
Restricted (Max Planck Institute of Colloids and Interfaces, MTKG; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Hinneburg, Hannes1, Author           
Korać, Petra, Author
Schirmeister, Falko1, Author           
Gasparov, Slavko, Author
Seeberger, Peter H.2, Author           
Zoldoš, Vlatka, Author
Kolarich, Daniel1, Author           
Affiliations:
1Daniel Kolarich, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863301              
2Peter H. Seeberger - Vaccine Development, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863308              

Content

show
hide
Free keywords: -
 Abstract: N- and O-glycans are attractive clinical biomarkers as glycosylation changes in response to diseases. The limited availability of defined clinical specimens impedes glyco-biomarker identification and validation in large patient cohorts. Formalin-fixed paraffin-embedded (FFPE) clinical specimens are the common form of sample preservation in clinical pathology, but qualitative and quantitative N- and O-glycomics of such samples has not been feasible to date. Here, we report a highly sensitive and glycan isomer selective method for simultaneous N- and O-glycomics from histopathological slides. As few as 2,000 cells isolated from FFPE tissue sections by laser capture microdissection were sufficient for in-depth histopathology-glycomics using porous graphitized carbon nanoLC ESI-MS/MS. N- and O-glycan profiles were similar between unstained and hematoxylin and eosin stained FFPE samples but differed slightly compared to fresh tissue. This method provides the key to unlock glyco-biomarker information from FFPE histopathological tissues archived in pathology laboratories worldwide.

Details

show
hide
Language(s):
 Dates: 2017-01-252017
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1074/mcp.M116.062414
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Molecular and Cellular Proteomics
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Bethesda, MD : American Society for Biochemistry and Molecular Biology
Pages: - Volume / Issue: 16 (4) Sequence Number: - Start / End Page: 524 - 536 Identifier: ISSN: 1535-9476