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  Unlocking cancer glycomes from histopathological formalin-fixed and paraffin-embedded (FFPE) tissue microdissections

Hinneburg, H., Korać, P., Schirmeister, F., Gasparov, S., Seeberger, P. H., Zoldoš, V., et al. (2017). Unlocking cancer glycomes from histopathological formalin-fixed and paraffin-embedded (FFPE) tissue microdissections. Molecular and Cellular Proteomics, 16(4), 524-536. doi:10.1074/mcp.M116.062414.

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Hinneburg, Hannes1, Author              
Korać, Petra, Author
Schirmeister, Falko1, Author              
Gasparov, Slavko, Author
Seeberger, Peter H.2, Author              
Zoldoš, Vlatka, Author
Kolarich, Daniel1, Author              
Affiliations:
1Daniel Kolarich, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863301              
2Peter H. Seeberger - Vaccine Development, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863308              

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 Abstract: N- and O-glycans are attractive clinical biomarkers as glycosylation changes in response to diseases. The limited availability of defined clinical specimens impedes glyco-biomarker identification and validation in large patient cohorts. Formalin-fixed paraffin-embedded (FFPE) clinical specimens are the common form of sample preservation in clinical pathology, but qualitative and quantitative N- and O-glycomics of such samples has not been feasible to date. Here, we report a highly sensitive and glycan isomer selective method for simultaneous N- and O-glycomics from histopathological slides. As few as 2,000 cells isolated from FFPE tissue sections by laser capture microdissection were sufficient for in-depth histopathology-glycomics using porous graphitized carbon nanoLC ESI-MS/MS. N- and O-glycan profiles were similar between unstained and hematoxylin and eosin stained FFPE samples but differed slightly compared to fresh tissue. This method provides the key to unlock glyco-biomarker information from FFPE histopathological tissues archived in pathology laboratories worldwide.

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 Dates: 2017-01-252017
 Publication Status: Published in print
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 Identifiers: DOI: 10.1074/mcp.M116.062414
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Title: Molecular and Cellular Proteomics
Source Genre: Journal
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Publ. Info: Bethesda, MD : American Society for Biochemistry and Molecular Biology
Pages: - Volume / Issue: 16 (4) Sequence Number: - Start / End Page: 524 - 536 Identifier: ISSN: 1535-9476