English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  High-flexibility combinatorial peptide synthesis with laser-based transfer of monomers in solid matrix material

Löffler, F., Foertsch, T. C., Popov, R., Mattes, D. S., Schlageter, M., Sedlmayr, M., et al. (2016). High-flexibility combinatorial peptide synthesis with laser-based transfer of monomers in solid matrix material. Nature Communications, 7: 11844. doi:10.1038/ncomms11844.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-5256-8 Version Permalink: http://hdl.handle.net/21.11116/0000-0006-78B9-3
Genre: Journal Article

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Löffler, Felix1, Author              
Foertsch, Tobias C., Author
Popov, Roman, Author
Mattes, Daniela S., Author
Schlageter, Martin, Author
Sedlmayr, Martyna, Author
Ridder, Barbara, Author
Dang, Florian-Xuan, Author
von Bojnicic-Kninski, Clemens, Author
Weber, Laura K., Author
Fischer, Andrea, Author
Greifenstein, Juliane, Author
Bykovskaya, Valentina, Author
Buliev, Ivan, Author
Bischoff, F. Ralf, Author
Hahn, Lothar, Author
Meier, Michael A. R., Author
Braese, Stefan, Author
Powell, Annie K., Author
Balaban, Teodor Silviu, Author
Breitling, Frank, AuthorNesterov-Mueller, Alexander, Author more..
Affiliations:
1External Organizations, ou_persistent22              

Content

show
hide
Free keywords: -
 Abstract: Laser writing is used to structure surfaces in many different ways in materials and life sciences. However, combinatorial patterning applications are still limited. Here we present a method for cost-efficient combinatorial synthesis of very-high-density peptide arrays with natural and synthetic monomers. A laser automatically transfers nanometre-thin solid material spots from different donor slides to an acceptor. Each donor bears a thin polymer film, embedding one type of monomer. Coupling occurs in a separate heating step, where the matrix becomes viscous and building blocks diffuse and couple to the acceptor surface. Furthermore, we can consecutively deposit two material layers of activation reagents and amino acids. Subsequent heat-induced mixing facilitates an in situ activation and coupling of the monomers. This allows us to incorporate building blocks with click chemistry compatibility or a large variety of commercially available non-activated, for example, posttranslationally modified building blocks into the array's peptides with 417,000 spots per cm(2).

Details

show
hide
Language(s):
 Dates: 2016
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: ISI: 000378678700001
DOI: 10.1038/ncomms11844
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Nature Communications
  Abbreviation : Nat. Commun.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: London : Nature Publishing Group
Pages: - Volume / Issue: 7 Sequence Number: 11844 Start / End Page: - Identifier: ISSN: 2041-1723