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  Purification of High-Complexity Peptide Microarrays by Spatially Resolved Array Transfer to Gold-Coated Membranes

Schirwitz, C., Löffler, F., Felgenhauer, T., Stadler, V., Nesterov-Mueller, A., Dahint, R., et al. (2013). Purification of High-Complexity Peptide Microarrays by Spatially Resolved Array Transfer to Gold-Coated Membranes. Advanced Materials, 25(11), 1598-1602. doi:10.1002/adma.201203853.

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Schirwitz, Christopher, Author
Löffler, Felix1, Author           
Felgenhauer, Thomas, Author
Stadler, Volker, Author
Nesterov-Mueller, Alexander, Author
Dahint, Reiner, Author
Breitling, Frank, Author
Bischoff, F. Ralf, Author
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1External Organizations, ou_persistent22              

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 Abstract: A method for the one-step purification of high-complexity peptide microarrays is presented. The entire peptide library is transferred from the synthesis support to a gold coated polyvinylidenfluoride (PVDF) membrane, whereby only full-length peptides covalently couple to the receptor membrane via an N-terminally added cysteine. Highly resolved peptide transfer and purification of up to 10 000 features per cm(2) is demonstrated.

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 Dates: 2013
 Publication Status: Published in print
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 Rev. Type: -
 Identifiers: ISI: 000316215700012
DOI: 10.1002/adma.201203853
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Title: Advanced Materials
  Other : Adv. Mater.
Source Genre: Journal
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Publ. Info: Weinheim : Wiley-VCH
Pages: - Volume / Issue: 25 (11) Sequence Number: - Start / End Page: 1598 - 1602 Identifier: ISSN: 0935-9648