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  Peptide Arrays with a Chip

Nesterov, A., Doersam, E., Cheng, Y.-C., Schirwitz, C., Maerkle, F., Löffler, F., et al. (2010). Peptide Arrays with a Chip. In Methods in Molecular Biology. Berlin: Springer.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-50EB-8 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-50EC-6
Genre: Book Chapter

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 Creators:
Nesterov, Alexander1, Author
Doersam, Edgar1, Author
Cheng, Yun-Chien1, Author
Schirwitz, Christopher1, Author
Maerkle, Frieder1, Author
Löffler, Felix, Author              
Koenig, Kai1, Author
Stadler, Volker1, Author
Bischoff, Ralf1, Author
Breitling, Frank1, Author
Affiliations:
1external, ou_persistent22              

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 Abstract: Today, lithographic methods enable combinatorial synthesis of >50,000 oligonucleotides per cm(2), an advance that has revolutionized the whole field of genomics. A similar development is expected for the field of proteomics, provided that affordable, very high-density peptide arrays are available. However, peptide arrays lag behind oligonucleotide arrays. This is mainly due to the monomer-by-monomer repeated consecutive coupling of 20 different amino acids associated with lithography, which adds up to an excessive number of coupling cycles. A combinatorial synthesis based on electrically charged solid amino acid particles resolves this problem. A computer chip consecutively addresses the different charged particles to a solid support, where, when completed, the whole layer of solid amino acid particles is melted at once. This frees hitherto immobilized amino acids to couple all 20 different amino acids in one single coupling reaction to the support. The method should allow for the translation of entire genomes into a set of overlapping peptides to be used in proteome research.

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 Dates: 2010
 Publication Status: Published in print
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Title: Methods in Molecular Biology
Source Genre: Series
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Publ. Info: Berlin : Springer
Pages: - Volume / Issue: 669 Sequence Number: - Start / End Page: - Identifier: ISSN: 1064-3745

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Title: Small Molecule Microarrays
Source Genre: Book
 Creator(s):
Uttamchandani, Mahesh, Editor
Yao, Shao Q., Editor
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-
Publ. Info: -
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: 109 - 124 Identifier: ISBN: 978-1-60761-844-7