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  Re-epithelialization of cutaneous wounds in adult zebrafish combines mechanisms of wound closure in embryonic and adult mammals

Richardson, R., Metzger, M., Knyphausen, P., Ramezani, T., Slanchev, K., Kraus, C., et al. (2016). Re-epithelialization of cutaneous wounds in adult zebrafish combines mechanisms of wound closure in embryonic and adult mammals. Development, 143(12), 2077-2088. doi:10.1242/dev.130492.

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http://dev.biologists.org/content/143/12/2077 (Publisher version)
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 Creators:
Richardson, Rebecca, Author
Metzger, Manuel, Author
Knyphausen, Philipp, Author
Ramezani, Thomas, Author
Slanchev, Krasimir1, Author              
Kraus, Christopher, Author
Schmelzer, Elmon, Author
Hammerschmidt, Matthias, Author
Affiliations:
1Department: Genes-Circuits-Behavior / Baier, MPI of Neurobiology, Max Planck Society, ou_1128545              

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Free keywords: EPITHELIAL-MESENCHYMAL TRANSITION; HUMAN KERATINOCYTE MIGRATION; GROWTH-FACTOR-BETA; TRANSGENIC ZEBRAFISH; CELL POLARITY; TGF-BETA; INFLAMMATORY RESPONSE; EXTENSION MOVEMENTS; INTEGRIN RECEPTORS; SIGNALING PATHWAYSDevelopmental Biology; FGF; Re-epithelialization; Skin; TGF-beta; Wound healing; Cell proliferation;
 Abstract: Re-epithelialization of cutaneous wounds in adult mammals takes days to complete and relies on numerous signalling cues and multiple overlapping cellular processes that take place both within the epidermis and in other participating tissues. Re-epithelialization of partial- or full-thickness skin wounds of adult zebrafish, however, is extremely rapid and largely independent of the other processes of wound healing. Live imaging after treatment with transgene-encoded or chemical inhibitors reveals that re-epithelializing keratinocytes repopulate wounds by TGF-beta- and integrin-dependent lamellipodial crawling at the leading edges of the epidermal tongue. In addition, re-epithelialization requires long-range epithelial rearrangements, involving radial intercalations, flattening and directed elongation of cells - processes that are dependent on Rho kinase, JNK and, to some extent, planar cell polarity within the epidermis. These rearrangements lead to a massive recruitment of keratinocytes from the adjacent epidermis and make re-epithelialization independent of keratinocyte proliferation and the mitogenic effect of FGF signalling, which are only required after wound closure, allowing the epidermis outside the wound to re-establish its normal thickness. Together, these results demonstrate that the adult zebrafish is a valuable in vivo model for studying and visualizing the processes involved in cutaneous wound closure, facilitating the dissection of direct from indirect and motogenic from mitogenic effects of genes and molecules affecting wound re-epithelialization.

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Language(s): eng - English
 Dates: 2016-06-01
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000390417400005
DOI: 10.1242/dev.130492
 Degree: -

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Title: Development
  Other : Development
Source Genre: Journal
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Publ. Info: Cambridge, Cambridgeshire : Company of Biologists
Pages: - Volume / Issue: 143 (12) Sequence Number: - Start / End Page: 2077 - 2088 Identifier: ISSN: 0950-1991
CoNE: https://pure.mpg.de/cone/journals/resource/954927546241