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  Mapping metabolites from rough terrain: laser ablation electrospray ionization on non-flat samples

Bartels, B., Kulkarni, P., Danz, N., Böcker, S., Saluz, H. P., & Svatoš, A. (2017). Mapping metabolites from rough terrain: laser ablation electrospray ionization on non-flat samples. RSC Advances, 7(15), 9045-9050. doi:10.1039/C6RA26854D.

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http://dx.doi.org/10.1039/C6RA26854D (Publisher version)
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 Creators:
Bartels, Benjamin1, 2, Author              
Kulkarni, Purva1, 2, Author              
Danz, Norbert, Author
Böcker, Sebastian, Author
Saluz, Hans Peter, Author
Svatoš, Aleš1, Author              
Affiliations:
1Research Group Mass Spectrometry, MPI for Chemical Ecology, Max Planck Society, ou_421899              
2IMPRS on Ecological Interactions, MPI for Chemical Ecology, Max Planck Society, ou_421900              

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 Abstract: Established laser-based ionization experiments require the surface of a sample to be as flat as possible to guarantee optimal laser focus. A laser ablation electrospray ionization (LAESI) source was custom-built to accommodate the topography of non-flat sample surfaces. Employing a confocal distance sensor, a height profile of the surface in question was recorded prior to the actual ionization experiment. The robustness of the system was evaluated by the metabolic profiling of radish (Raphanus sativus) leaves, chosen due to their pronounced surface features and known content of specialized metabolites. After the ionization experiments, light microscopy imaging was performed to evaluate ablation crater size and position. Reproducible ablation mark diameters of 69 7 mm in average have been achieved. Mass spectrometric imaging capability has been proven on R. sativus leaf samples as well.

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 Dates: 2017-01-122017-01-27
 Publication Status: Published online
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 Identifiers: Other: MS182
DOI: 10.1039/C6RA26854D
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Title: RSC Advances
  Abbreviation : RSC Adv.
Source Genre: Journal
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Publ. Info: Cambridge, UK : Royal Society of Chemistry
Pages: - Volume / Issue: 7 (15) Sequence Number: - Start / End Page: 9045 - 9050 Identifier: ISSN: 2046-2069
CoNE: https://pure.mpg.de/cone/journals/resource/2046-2069