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  Particle-Based Microarrays of Oligonucleotides and Oligopeptides

Nesterov-Mueller, A., Maerkle, F., Hahn, L., Foertsch, T., Schillo, S., Bykovskaya, V., et al. (2014). Particle-Based Microarrays of Oligonucleotides and Oligopeptides. Microarrays, 3(4), 245-262. doi:10.3390/microarrays3040245.

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Nesterov-Mueller, Alexander, Author
Maerkle, Frieder, Author
Hahn, Lothar, Author
Foertsch, Tobias, Author
Schillo, Sebastian, Author
Bykovskaya, Valentina, Author
Sedlmayr, Martyna, Author
Weber, Laura K, Author
Ridder, Barbara, Author
Soehindrijo, Miriam, Author
Muenster, Bastian, Author
Striffler, Jakob, Author
Bischoff, F Ralf, Author
Breitling, Frank, Author
Löffler, Felix F.1, Author              
Roth, Günter, Contributor
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1External Organizations, ou_persistent22              

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 Abstract: In this review, we describe different methods of microarray fabrication based on the use of micro-particles/-beads and point out future tendencies in the development of particle-based arrays. First, we consider oligonucleotide bead arrays, where each bead is a carrier of one specific sequence of oligonucleotides. This bead-based array approach, appearing in the late 1990s, enabled high-throughput oligonucleotide analysis and had a large impact on genome research. Furthermore, we consider particle-based peptide array fabrication using combinatorial chemistry. In this approach, particles can directly participate in both the synthesis and the transfer of synthesized combinatorial molecules to a substrate. Subsequently, we describe in more detail the synthesis of peptide arrays with amino acid polymer particles, which imbed the amino acids inside their polymer matrix. By heating these particles, the polymer matrix is transformed into a highly viscous gel, and thereby, imbedded monomers are allowed to participate in the coupling reaction. Finally, we focus on combinatorial laser fusing of particles for the synthesis of high-density peptide arrays. This method combines the advantages of particles and combinatorial lithographic approaches.

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 Dates: 2014-12
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.3390/microarrays3040245
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Title: Microarrays
  Alternative Title : Microarrays (Basel)
Source Genre: Journal
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Publ. Info: MDPI
Pages: - Volume / Issue: 3 (4) Sequence Number: - Start / End Page: 245 - 262 Identifier: ISSN: 2076-3905