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  Time lapse in vivo visualization of developmental stabilization of synaptic receptors at Neuromuscular Junctions

Yampolsky, P., Pacifici, P. G., Lomb, L., Giese, G., Rudolf, R., Roeder, I. V., et al. (2010). Time lapse in vivo visualization of developmental stabilization of synaptic receptors at Neuromuscular Junctions. The Journal of Biological Chemistry, 285(45), 34589-34596. doi:10.1074/jbc.M110.168880.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-5F59-7 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-5F5A-5
Genre: Journal Article
Alternative Title : Time lapse in vivo visualization of developmental stabilization of synaptic receptors at Neuromuscular Junctions

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 Creators:
Yampolsky, Pessah1, Author              
Pacifici, Pier Giorgio1, Author              
Lomb, Lukas2, Author              
Giese, Günter3, Author              
Rudolf, Rüdiger, Author
Roeder, Ira V., Author
Witzemann, Veit1, Author              
Affiliations:
1Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497704              
2Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              
3Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497699              

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 Abstract: The lifetime of nicotinic acetylcholine receptors (AChRs) in neuromuscular junctions (NMJs) is increased from <1 day to >1 week during early postnatal development. However, the exact timing of AChR stabilization is not known, and its correlation to the concurrent embryonic to adult AChR channel conversion, NMJ remodeling, and neuromuscular diseases is unclear. Using a novel time lapse in vivo imaging technology we show that replacement of the entire receptor population of an individual NMJ occurs end plate-specifically within hours. This makes it possible to follow directly in live animals changing stabilities of end plate receptors. In three different, genetically modified mouse models we demonstrate that the metabolic half-life values of synaptic AChRs increase from a few hours to several days after postnatal day 6. Developmental stabilization is independent of receptor subtype and apparently regulated by an intrinsic muscle-specific maturation program. Myosin Va, an F-actin-dependent motor protein, is also accumulated synaptically during postnatal development and thus could mediate the stabilization of end plate AChR.

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Language(s): eng - English
 Dates: 2010-07-272010-09-022010-09-022010-11-05
 Publication Status: Published in print
 Pages: 8
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 Rev. Method: Peer
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Title: The Journal of Biological Chemistry
  Other : JBC
Source Genre: Journal
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Publ. Info: Baltimore, etc. : American Society for Biochemistry and Molecular Biology [etc.]
Pages: - Volume / Issue: 285 (45) Sequence Number: - Start / End Page: 34589 - 34596 Identifier: ISSN: 0021-9258
CoNE: https://pure.mpg.de/cone/journals/resource/954925410826_1