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  Visualization of Cellular Components in a Mammalian Cell with Liquid-Cell Transmission Electron Microscopy

Besztejan, S., Keskin, S., Manz, S., Kassier, G., Bücker, R., Venegas-Rojas, D., et al. (2017). Visualization of Cellular Components in a Mammalian Cell with Liquid-Cell Transmission Electron Microscopy. Microscopy and Microanalysis, 23(1), 46-55. doi:10.1017/S1431927616012708.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-8016-7 Version Permalink: http://hdl.handle.net/21.11116/0000-0001-E1A2-9
Genre: Journal Article

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visualization_of_cellular_components_in_a_mammalian_cell_with_liquidcell_transmission_electron_microscopy.pdf (Publisher version), 738KB
 
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https://dx.doi.org/10.1017/S1431927616012708 (Publisher version)
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 Creators:
Besztejan, Stephanie1, 2, Author              
Keskin, Sercan3, Author              
Manz, Stephanie3, Author              
Kassier, Günther3, Author              
Bücker, Robert3, Author              
Venegas-Rojas, Deybith4, Author
Trieu, Hoc K.4, Author
Rentmeister , Andrea5, Author
Miller, R. J. Dwayne3, 6, Author              
Affiliations:
1University of Hamburg, External Organizations, ou_2035287              
2The Hamburg Centre for Ultrafast Imaging, University of Hamburg, ou_persistent22              
3Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society, ou_1938288              
4Institute of Microsystems Technology, Hamburg University of Technology (TUHH), ou_persistent22              
5Institute of Biochemistry, Westfälische Wilhelms-Universität Münster, ou_persistent22              
6Departments of Chemistry and Physics, University of Toronto, ou_persistent22              

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Free keywords: Liquid-cell TEM, mammalian cells, nanofluidic cell, in situ imaging, gold nanoparticles
 Abstract: We present liquid-cell transmission electron microscopy (liquid-cell TEM) imaging of fixed and non-fixed prostate cancer cells (PC3 and LNCaP) with high resolution in a custom developed silicon nitride liquid cell. Fixed PC3 cells were imaged for 90–120 min without any discernable damage. High contrast on the cellular structures was obtained even at low electron doses (~2.5 e−/nm2 per image). The images show distinct structures of cell compartments (nuclei and nucleoli) and cell boundaries without any further sample embedding, dehydration, or staining. Furthermore, we observed dynamics of vesicles trafficking from the cell membrane in consecutive still frames in a non-fixed cell. Our findings show that liquid-cell TEM, operated at low electron dose, is an excellent tool to investigate dynamic events in non-fixed cells with enough spatial resolution (few nm) and natural amplitude contrast to follow key intracellular processes.

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Language(s): eng - English
 Dates: 2016-08-122016-12-142017-01-312017-02
 Publication Status: Published in print
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1017/S1431927616012708
 Degree: -

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Title: Microscopy and Microanalysis
Source Genre: Journal
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Publ. Info: Cambridge, United Kingdom : Cambridge University Press
Pages: - Volume / Issue: 23 (1) Sequence Number: - Start / End Page: 46 - 55 Identifier: -