English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Formation of focal adhesion-stress fibre complexes coordinated by adhesive and non-adhesive surface domains

Zimerman, B., Arnold, M., Ulmer, J., Blümmel, J., Besser, A., Spatz, J. P., et al. (2004). Formation of focal adhesion-stress fibre complexes coordinated by adhesive and non-adhesive surface domains. IEE Proceedings-Nanobiotechnology, 151(2), 62-66. doi:10.1049/ip-nbt:20040474.

Item is

Files

show Files
hide Files
:
IEEProcNanobiotechnol_151_2004_62.pdf (Any fulltext), 2MB
 
File Permalink:
-
Name:
IEEProcNanobiotechnol_151_2004_62.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Description:
-
Description:
-

Creators

show
hide
 Creators:
Zimerman, B, Author
Arnold, Marco1, Author              
Ulmer, Jens1, Author              
Blümmel, Jacques1, Author              
Besser, Achim, Author
Spatz, Joachim P.1, 2, Author              
Geiger, Benjamin, Author
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany, ou_persistent22              

Content

show
hide
Free keywords: -
 Abstract: Cell motility consists of repeating cycles of protrusion of a leading edge in the direction of migration, attachment of the advancing membrane to the matrix, and pulling of the trailing edge forward. In this dynamic process there is a major role for the cytoskeleton, which drives the protrusive events via polymerisation of actin in the lamellipodium, followed by actomyosin contractility. To study the transition of the actin cytoskeleton from a 'protrusive' to 'retractive' form, we have monitored the formation of focal adhesions and stress fibres during cell migration on a micro-patterned surface. This surface consisted of parallel arrays of 2 microm-wide, fibronectin-coated gold stripes, separated by non-adhesive (poly(ethylene glycol)-coated) glass areas with variable width, ranging from 4-12 microm. Monitoring the spreading of motile cells indicated that cell spreading was equally effective along and across the adhesive stripes, as long as the non-adhesive spaces between them did not exceed 6 microm. When the width of the PEG region was 8 microm or more, cells became highly polarised upon spreading, and failed to reach the neighboring adhesive stripes. It was also noted that as soon as the protruding lamella successfully crossed the PEG-coated area and reached an adhesive region, the organisation of actin in that area was transformed from a diffuse meshwork into a bundle, oriented perpendicularly to the stripes and anchored at its ends in focal adhesions. This transition depends on actomyosin-based contractility and is apparently triggered by the adhesion to the rigid fibronectin surface.

Details

show
hide
Language(s): eng - English
 Dates: 2004-02-262003-10-012004-04-01
 Publication Status: Published in print
 Pages: 5
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: IEE Proceedings-Nanobiotechnology
  Other : IEE Proc-Nanobiotechnol.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: [Stevenage, U.K. : Institution of Electrical Engineers
Pages: - Volume / Issue: 151 (2) Sequence Number: - Start / End Page: 62 - 66 Identifier: ISSN: 1478-1581
CoNE: https://pure.mpg.de/cone/journals/resource/1000000000278590