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  Getting a grip: hyaluronan-mediated cellular adhesion

Curtis, J. E., & Spatz, J. P. (2004). Getting a grip: hyaluronan-mediated cellular adhesion. In Optical Trapping and Optical Micromanipulation, Proc. SPIE, Vol. 5514 (pp. 455-466). Birmingham: SPIE.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-34AB-2 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002E-8B6B-7
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 Creators:
Curtis, Jennifer E.1, Author              
Spatz, Joachim P.1, 2, Author              
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany, ou_persistent22              

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Free keywords: holographic optical tweezers, calibration, hyaluronan, cell adhesion, pericellular matrix, chondrocytes, membrane tethers
 Abstract: Holographic optical tweezers (HOTs) techniques are further developed to study hyaluronan-mediated adhesion of chondrocyte cells. We present a calibration scheme and address fundamental issues concerning the use of HOTs for quantitative force measurements. Influence of SLM pixelation on trap stiffness is observed and can be utilized to design calibrated HOTs more effectively. It is also shown that the HOTs trapping stiffness can vary significantly over short distances. Then we use HOTs cell adhesion assays investigate the viscoelastic and adhesive nature of chondrocytes' pericellular matrix (PCM) at two different time steps (30 minute and 24 hour incubation periods). Surprisingly, no physical influence of the large, presumably gel-like PCM is observed. However, a difference is discerned in the adhesiveness of the two sets of cells. The early-stage cells have reversible adhesion with negatively-charged and fibronectin-coated microspheres even after they are held at the cell surface for 10 seconds. In contrast, late stage cells stick irreversibly to all types of beads: positive, negative, fibronectin and hyaluronan-coated. Additionally, only the late stage cells produce membrane tethers. These observations suggest that the late-stage chondrocytes have less surface-associated hyaluronan and have interesting implications for the role of hyaluronan in the early stages of cell adhesion.

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Language(s): eng - English
 Dates: 2004-10-18
 Publication Status: Published in print
 Pages: 12
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 Table of Contents: -
 Rev. Method: -
 Identifiers: DOI: 10.1117/12.560049
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Title: Optical Trapping and Optical Micromanipulation
Place of Event: Denver, CO
Start-/End Date: 2004-08-02 - 2004-08-02

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Title: Optical Trapping and Optical Micromanipulation, Proc. SPIE, Vol. 5514
Source Genre: Proceedings
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Publ. Info: Birmingham : SPIE
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: 455 - 466 Identifier: ISBN: 9780819454522

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Title: Proceedings SPIE
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Publ. Info: Birmingham : SPIE
Pages: - Volume / Issue: 1554 Sequence Number: - Start / End Page: - Identifier: ISSN: 0277-786X