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  Expression of synthetic genes encoding bovine and human basic fibroblast growth factors (bFGFs) in Escherichia coli.

Knoerzer, W., Binder, H. P., Schneider, K., Gruß, P., McCarthy, J. E. G., & Risau, W. (1989). Expression of synthetic genes encoding bovine and human basic fibroblast growth factors (bFGFs) in Escherichia coli. Gene, 75(1), 21-30. doi:10.1016/0378-1119(89)90379-X.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-6392-6 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-6395-F
Genre: Journal Article

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2395608.pdf (Publisher version), 2MB
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 Creators:
Knoerzer, W., Author
Binder, H. P., Author
Schneider, K., Author
Gruß, P.1, Author              
McCarthy, J. E. G., Author
Risau, W., Author
Affiliations:
1Department of Molecular Cell Biology, MPI for biophysical chemistry, Max Planck Society, ou_578585              

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 Abstract: Synthetic genes encoding bovine and human basic fibroblast growth factors (bFGFs) were assembled and cloned using established Escherichia coli expression plasmids. Transformed E. coli cells were able to synthesize either a fusion protein, comprising the first seven amino acids of β-galactosidase, a linker fragment and bovine FGF, or genomic human bFGF. The two growth factors were purified from E. coli lysates by cation exchange and heparin-Sepharose affinity chromatography. The purified recombinant proteins were biologically active as monitored by their mitogenic activity for bovine aortic endothelial cells and their angiogenic capacity in the rabbit cornea.

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Language(s): eng - English
 Dates: 1989-01
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1016/0378-1119(89)90379-X
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Title: Gene
Source Genre: Journal
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Pages: - Volume / Issue: 75 (1) Sequence Number: - Start / End Page: 21 - 30 Identifier: -