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  Visualization of Cellular Components in a Mammalian Cell with Liquid-Cell Transmission Electron Microscopy

Besztejan, S., Keskin, S., Manz, S., Kassier, G., Bücker, R., Venegas-Rojas, D., et al. (2017). Visualization of Cellular Components in a Mammalian Cell with Liquid-Cell Transmission Electron Microscopy. Microscopy and Microanalysis. doi:10.1017/S1431927616012708.

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Datensatz-Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-7E14-9 Versions-Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-7E15-7
Genre: Zeitschriftenartikel

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externe Referenz:
https://doi.org/10.1017/S1431927616012708 (Verlagsversion)
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Urheber

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 Urheber:
Besztejan, Stephanie1, 2, 3, Autor              
Keskin, Sercan1, 4, Autor              
Manz, Stephanie4, Autor
Kassier, Günther4, Autor
Bücker, Robert4, Autor
Venegas-Rojas, Deybith5, Autor
Trieu, Hoc K.5, Autor
Rentmeister, Andrea6, Autor
Miller, R. J. Dwayne3, 4, 7, Autor
Affiliations:
1International Max Planck Research School for Ultrafast Imaging & Structural Dynamics (IMPRS-UFAST), Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society, ou_2266714              
2Chemistry Department, Institute for Biochemistry and Molecular Biology, University of Hamburg, Martin-Luther-King Platz 6, 20146 Hamburg, Germany, ou_persistent22              
3The Hamburg Centre for Ultrafast Imaging, University of Hamburg, Luruper Chaussee 149, 22761 Hamburg, Germany, ou_persistent22              
4Max Planck Institute for the Structure and Dynamics of Matter, Luruper Chaussee 149, Geb. 99 (CFEL), 22761 Hamburg, Germany, ou_persistent22              
5Institute of Microsystems Technology, Hamburg University of Technology (TUHH), Eißendorfer Straße 42, 21073 Hamburg, Germany, ou_persistent22              
6Institute of Biochemistry, Westfälische Wilhelms-Universität Münster, Wilhelm-Klemm-Strasse 2, 48149 Muenster, Germany, ou_persistent22              
7Departments of Chemistry and Physics, University of Toronto, 80 St George St, Toronto, ON, CanadaM5S3H6, ou_persistent22              

Inhalt

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Schlagwörter: Liquid-cell TEM, mammalian cells, nanofluidic cell, in situ imaging, gold nanoparticles
 Zusammenfassung: We present liquid-cell transmission electron microscopy (liquid-cell TEM) imaging of fixed and non-fixed prostate cancer cells (PC3 and LNCaP) with high resolution in a custom developed silicon nitride liquid cell. Fixed PC3 cells were imaged for 90–120 min without any discernable damage. High contrast on the cellular structures was obtained even at low electron doses (~2.5 e-/nm2 per image). The images show distinct structures of cell compartments (nuclei and nucleoli) and cell boundaries without any further sample embedding, dehydration, or staining. Furthermore, we observed dynamics of vesicles trafficking from the cell membrane in consecutive still frames in a non-fixed cell. Our findings show that liquid-cell TEM, operated at low electron dose, is an excellent tool to investigate dynamic events in non-fixed cells with enough spatial resolution (few nm) and natural amplitude contrast to follow key intracellular processes.

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Sprache(n): eng - Englisch
 Datum: 2016-08-122016-12-142017-01-31
 Publikationsstatus: Online publiziert
 Seiten: 10
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.1017/S1431927616012708
 Art des Abschluß: -

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Quelle 1

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Titel: Microscopy and Microanalysis
  Kurztitel : Microsc. Microanal.
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: New York, NY : Cambridge University Press
Seiten: - Band / Heft: - Artikelnummer: - Start- / Endseite: - Identifikator: ISSN: 1431-9276
CoNE: /journals/resource/991042731793414