English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  On the diagnosis of erythrocyte enzyme defects in the presence of high reticulocyte counts.

Lakomek, M., Schröter, W., De Mayer, G., & Winkler, A. H. (1989). On the diagnosis of erythrocyte enzyme defects in the presence of high reticulocyte counts. British Journal of Haematology, 72(3), 445-451. doi:10.1111/j.1365-2141.1989.tb07730.x.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-8F78-2 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-8F7A-D
Genre: Journal Article

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Lakomek, M., Author
Schröter, W., Author
De Mayer, G., Author
Winkler, A. H.1, Author              
Affiliations:
1Abteilung Biochemische Kinetik, MPI for biophysical chemistry, Max Planck Society, ou_578616              

Content

show
hide
Free keywords: -
 Abstract: Summary. The separation of red blood cells into reticulocytes and young and old erythrocytes enables investigations of fractions with different contents of reticulocytes. Activities of hexokinase, glucose phosphate isomerase, phosphofructokinase, pyruvate kinase and glucose-6-phosphate dehydrogenase showed a linear relationship to reticulocyte counts. The dependence of these enzyme activities on the age of the red blood cells exhibited a strong decline from the reticulocyte to the young erythrocyte stage followed by only little further loss of activity, thus leading to a biphasic decay of enzyme activities. By linear regression analysis enzyme activities in erythrocytes (AE) and reticulocytes (AR) could be evaluated. The activity of a given enzyme in the reticulocyte exceeded that of the erythrocyte; the quotient AR/AE represents the decline of enzyme activity from the reticulocyte to the erythrocyte stage. This value AR/AE is 16·7 for pyruvate kinase and 9·4 for hexokinase and thus considerably higher than that for the other enzymes investigated (glucose phosphate isomerase: 2·9, phosphofructokinase: 4·3, glucose-6-phosphate dehydrogenase: 4·5). In patients suffering from erythrocyte enzymopathies, the AR/AE for pyruvate kinase was 16·2 and thus almost identical to the normal enzyme. Calibration curves where the enzyme activity is plotted versus the fraction of reticulocytes enable the determination of normal activity of a given erythrocyte enzyme depending on the content of reticulocytes in red blood cell suspensions. Thus an unambiguous diagnosis of enzyme defects irrespective of reticulocyte counts becomes possible.

Details

show
hide
Language(s): eng - English
 Dates: 1989-07
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: British Journal of Haematology
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 72 (3) Sequence Number: - Start / End Page: 445 - 451 Identifier: -