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  Phylointeractomics reconstructs functional evolution of protein binding

Kappei, D., Scheibe, M., Paszkowski-Rogacz, M., Bluhm, A., Gossmann, T. I., Dietz, S., et al. (2017). Phylointeractomics reconstructs functional evolution of protein binding. Nature Communications, 8: 14334. doi:10.1038/ncomms14334.

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 Creators:
Kappei, Dennis1, Author
Scheibe, Marion2, Author              
Paszkowski-Rogacz, Maciej1, Author
Bluhm, Alina1, Author
Gossmann, Toni Ingolf1, Author
Dietz, Sabrina1, Author
Dejung, Mario1, Author
Herlyn, Holger1, Author
Buchholz, Frank1, Author
Mann, Matthias2, Author              
Butter, Falk1, Author
Affiliations:
1external, ou_persistent22              
2Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565159              

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Free keywords: TELOMERE MAINTENANCE; AFFINITY PURIFICATION; MAMMALIAN TELOMERES; TRF1; RECRUITMENT; COMPLEX; DNA; IDENTIFICATION; EFFICIENT; PROTOCOLScience & Technology - Other Topics;
 Abstract: Molecular phylogenomics investigates evolutionary relationships based on genomic data. However, despite genomic sequence conservation, changes in protein interactions can occur relatively rapidly and may cause strong functional diversification. To investigate such functional evolution, we here combine phylogenomics with interaction proteomics. We develop this concept by investigating the molecular evolution of the shelterin complex, which protects telomeres, across 16 vertebrate species from zebrafish to humans covering 450 million years of evolution. Our phylointeractomics screen discovers previously unknown telomere-associated proteins and reveals how homologous proteins undergo functional evolution. For instance, we show that TERF1 evolved as a telomere-binding protein in the common stem lineage of marsupial and placental mammals. Phylointeractomics is a versatile and scalable approach to investigate evolutionary changes in protein function and thus can provide experimental evidence for phylogenomic relationships.

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Language(s): eng - English
 Dates: 2016-12-162017-02-08
 Publication Status: Published online
 Pages: 9
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000393601600001
DOI: 10.1038/ncomms14334
 Degree: -

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Title: Nature Communications
  Abbreviation : Nat. Commun.
Source Genre: Journal
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Publ. Info: London : Nature Publishing Group
Pages: - Volume / Issue: 8 Sequence Number: 14334 Start / End Page: - Identifier: ISSN: 2041-1723
CoNE: https://pure.mpg.de/cone/journals/resource/2041-1723