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  Osmoregulation in the Halophilic Bacterium Halomonas elongata: A Case Study for Integrative Systems Biology

Kindzierski, V., Raschke, S., Knabe, N., Siedler, F., Scheffer, B., Pflueger-Grau, K., et al. (2017). Osmoregulation in the Halophilic Bacterium Halomonas elongata: A Case Study for Integrative Systems Biology. PLoS One, 12(1): e0168818. doi:10.1371/journal.pone.0168818.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-9D5B-E Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-9D5C-C
Genre: Journal Article

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 Creators:
Kindzierski, Viktoria1, Author
Raschke, Silvia1, Author
Knabe, Nicole1, Author
Siedler, Frank2, Author              
Scheffer, Beatrix2, Author              
Pflueger-Grau, Katharina1, Author
Pfeiffer, Friedhelm2, Author              
Oesterhelt, Dieter2, Author              
Marin-Sanguino, Alberto1, Author
Kunte, Hans-Joerg1, Author
Affiliations:
1external, ou_persistent22              
2Oesterhelt, Dieter / Membrane Biochemistry, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565164              

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Free keywords: ECTOINE TRANSPORTER TEAABC; BINDING-PROTEIN TEAA; ESCHERICHIA-COLI; COMPATIBLE SOLUTE; ALANINE DEHYDROGENASE; GENE-CLUSTER; DSM 2581(T); AMINO-ACID; BIOSYNTHESIS; ACCUMULATIONScience & Technology - Other Topics;
 Abstract: Halophilic bacteria use a variety of osmoregulatory methods, such as the accumulation of one or more compatible solutes. The wide diversity of compounds that can act as compatible solute complicates the task of understanding the different strategies that halophilic bacteria use to cope with salt. This is specially challenging when attempting to go beyond the pathway that produces a certain compatible solute towards an understanding of how the metabolic network as a whole addresses the problem. Metabolic reconstruction based on genomic data together with Flux Balance Analysis (FBA) is a promising tool to gain insight into this problem. However, as more of these reconstructions become available, it becomes clear that processes predicted by genome annotation may not reflect the processes that are active in vivo. As a case in point, E. coli is unable to grow aerobically on citrate in spite of having all the necessary genes to do it. It has also been shown that the realization of this genetic potential into an actual capability to metabolize citrate is an extremely unlikely event under normal evolutionary conditions. Moreover, many marine bacteria seem to have the same pathways to metabolize glucose but each species uses a different one. In this work, a metabolic network inferred from genomic annotation of the halophilic bacterium Halomonas elongata and proteomic profiling experiments are used as a starting point to motivate targeted experiments in order to find out some of the defining features of the osmoregulatory strategies of this bacterium. This new information is then used to refine the network in order to describe the actual capabilities of H. elongata, rather than its genetic potential.

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Language(s): eng - English
 Dates: 2017-01-12
 Publication Status: Published online
 Pages: 22
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
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Title: PLoS One
Source Genre: Journal
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Publ. Info: San Francisco, CA : Public Library of Science
Pages: - Volume / Issue: 12 (1) Sequence Number: e0168818 Start / End Page: - Identifier: ISSN: 1932-6203
CoNE: https://pure.mpg.de/cone/journals/resource/1000000000277850