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  Using the Volta phase plate with defocus for cryo-EM single particle analysis.

Danev, R., Tegunov, D., & Baumeister, W. (2017). Using the Volta phase plate with defocus for cryo-EM single particle analysis. eLife, 6: e23006. doi:10.7554/eLife.23006.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-9FB4-3 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-9FBA-8
Genre: Journal Article

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e23006-download.pdf (Publisher version), 3MB
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© 2017, Danev et al This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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 Creators:
Danev, Radostin1, Author              
Tegunov, Dmitry2, Author              
Baumeister, Wolfgang1, Author              
Affiliations:
1Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565142              
2Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1863498              

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 Abstract: Previously, we reported an in-focus data acquisition method for cryo-EM single-particle analysis with the Volta phase plate (Danev and Baumeister, 2016). Here, we extend the technique to include a small amount of defocus which enables contrast transfer function measurement and correction. This hybrid approach simplifies the experiment and increases the data acquisition speed. It also removes the resolution limit inherent to the in-focus method thus allowing 3D reconstructions with resolutions better than 3 angstrom.

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Language(s): eng - English
 Dates: 2017-01-21
 Publication Status: Published online
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 Rev. Method: Peer
 Identifiers: DOI: 10.7554/eLife.23006
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Title: eLife
Source Genre: Journal
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Pages: 9 Volume / Issue: 6 Sequence Number: e23006 Start / End Page: - Identifier: -