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  Stereotaxic gene delivery in the rodent brain

Cetin, A., Komai, S., Eliava, M., Seeburg, P. H., & Osten, P. (2006). Stereotaxic gene delivery in the rodent brain. Nature Protocols, 1(6), 3166-3173. doi:10.1038/nprot.2006.450.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-A73D-0 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-A73E-E
Genre: Journal Article
Alternative Title : Stereotaxic gene delivery in the rodent brain

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NatProt_1_2006_3166.pdf (Any fulltext), 9MB
 
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 Creators:
Cetin, Ali1, Author              
Komai, Shoji2, Author              
Eliava, Marina1, Author              
Seeburg, Peter H.1, Author              
Osten, Pavel1, Author              
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1Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497704              
2Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497699              

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 Abstract: Stereotaxic surgery has been an invaluable tool in systems neuroscience, applied in many experiments for the creation of site-targeted lesions, injection of anatomical tracers or implantation of electrodes or microdialysis probes. In this protocol, we describe stereotaxic surgery optimized for gene delivery by recombinant adeno-associated viruses and lentiviruses in mice and rats. This method allows the manipulation of gene expression in the rodent brain with excellent spatiotemporal control; essentially any brain region of choice can be targeted and cells (or a subpopulation of cells) in that region can be stably genetically altered at any postnatal developmental stage up to adulthood. Many aspects of the method, its versatility, ease of application and high reproducibility, make it an attractive approach for studying genetic, cellular and circuit functions in the brain. The entire protocol can be completed in 1-2 hours.

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Language(s): eng - English
 Dates: 200620062007-01-312006
 Publication Status: Published in print
 Pages: 9
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 Rev. Type: Peer
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Title: Nature Protocols
  Other : Nat. Protoc.
Source Genre: Journal
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Pages: - Volume / Issue: 1 (6) Sequence Number: - Start / End Page: 3166 - 3173 Identifier: ISSN: 1750-2799
CoNE: https://pure.mpg.de/cone/journals/resource/1000000000223800_1