English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
Add to Basket
 Local TagsRelease HistoryDetailsSummary
  High content image analysis of focal adhesion-dependent mechanosensitive stem cell differentiation

Holle, A. W., McIntyre, A. J., Kehe, J., Wijesekara, P., Young, J. L., Vincent, L. G., et al. (2016). High content image analysis of focal adhesion-dependent mechanosensitive stem cell differentiation. Integrative Biology, 8(10), 1049-1058. doi:10.1039/C6IB00076B.

Item is

 

show all hide all

Basic

show hide
Item Permalink: https://hdl.handle.net/11858/00-001M-0000-002C-A9E3-8 Version Permalink: https://hdl.handle.net/11858/00-001M-0000-002E-2F8F-2
Genre: Journal Article

Files

show Files
hide Files
:
IntegrBiol_8_2016_1049.pdf (Any fulltext), 4MB
 
File Permalink:
-
Name:
IntegrBiol_8_2016_1049.pdf
Description:
-
OA-Status:
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
IntegrBiol_8_2016_1049_Suppl1.pdf (Supplementary material), 974KB
 
File Permalink:
-
Name:
IntegrBiol_8_2016_1049_Suppl1.pdf
Description:
-
OA-Status:
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
IntegrBiol_8_2016_1049_Suppl2.xlsx (Supplementary material), 320KB
 
File Permalink:
-
Name:
IntegrBiol_8_2016_1049_Suppl2.xlsx
Description:
-
OA-Status:
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Locator:
http://pubs.rsc.org/en/content/articlepdf/2016/ib/c6ib00076b (Any fulltext)
Description:
-
OA-Status:
Locator:
http://www.rsc.org/suppdata/c6/ib/c6ib00076b/c6ib00076b1.pdf (Any fulltext)
Description:
-
OA-Status:
Locator:
http://www.rsc.org/suppdata/c6/ib/c6ib00076b/c6ib00076b2.xlsx (Any fulltext)
Description:
-
OA-Status:

Creators

show
hide
 Creators:
Holle, Andrew W., Author
McIntyre, Alistair J., Author
Kehe, Jared, Author
Wijesekara, Piyumi, Author
Young, Jennifer L.1, Author           
Vincent, Ludovic G., Author
Engler, Adam J., Author
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              

Content

show
hide
Free keywords: -
 Abstract: Human mesenchymal stem cells (hMSCs) receive differentiation cues from a number of stimuli, including extracellular matrix (ECM) stiffness. The pathways used to sense stiffness and other physical cues are just now being understood and include proteins within focal adhesions. To rapidly advance the pace of discovery for novel mechanosensitive proteins, we employed a combination of in silico and high throughput in vitro methods to analyze 47 different focal adhesion proteins for cryptic kinase binding sites. High content imaging of hMSCs treated with small interfering RNAs for the top 6 candidate proteins showed novel effects on both osteogenic and myogenic differentiation; Vinculin and SORBS1 were necessary for stiffness-mediated myogenic and osteogenic differentiation, respectively. Both of these proteins bound to MAPK1 (also known as ERK2), suggesting that it plays a context-specific role in mechanosensing for each lineage; validation for these sites was performed. This high throughput system, while specifically built to analyze stiffness-mediated stem cell differentiation, can be expanded to other physical cues to more broadly assess mechanical signaling and increase the pace of sensor discovery.

Details

show
hide
Language(s): eng - English
 Dates: 2016-10-10
 Publication Status: Issued
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1039/C6IB00076B
URI: https://www.ncbi.nlm.nih.gov/pubmed/27723854
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Integrative Biology
  Abbreviation : Integr. Biol.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Cambridge, England : Royal Society of Chemistry
Pages: - Volume / Issue: 8 (10) Sequence Number: - Start / End Page: 1049 - 1058 Identifier: ISSN: 1757-9694
CoNE: https://pure.mpg.de/cone/journals/resource/1757-9694