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  Cardiac-specific activation of Cre expression at late fetal development

Opherk, J.-P., Yampolsky, P., Hardt, S. E., Schoels, W., Katus, H. A., Koenen, M., et al. (2007). Cardiac-specific activation of Cre expression at late fetal development. Biochemical and Biophysical Research Communications, 359(2), 209-213. doi:10.1016/j.bbrc.2007.05.045.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-ACC4-7 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-ACC5-5
Genre: Journal Article
Alternative Title : Cardiac-specific activation of Cre expression at late fetal development

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BiochemBiophysResComm_359_2007_209.pdf (Any fulltext), 3MB
 
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 Creators:
Opherk, Jan-Patrick1, Author              
Yampolsky, Pessah1, 2, Author              
Hardt, Stefan E., Author
Schoels, Wolfgang, Author
Katus, Hugo A., Author
Koenen, Michael1, Author              
Zehelein, Jörg1, 2, Author              
Affiliations:
1Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497701              
2Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497704              

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Free keywords: Cardiac Troponin I promoter; Cre recombinase; Conditional gene targeting; Cardiac development; Cardiovascular disease; Animal models of human disease
 Abstract: In a first step towards dissecting molecular mechanisms that contribute to the development of cardiac diseases, we have generated transgenic mice that express a Cre-GFP fusion protein under the transcriptional control of a 4.3kb murine cardiac Troponin I gene (cTnI) promoter. Cre-GFP expression, similar in three transgenic lines, is described in one line. In mouse embryos, transgenic for the Cre-GFP and ROSA lacZ reporter allele, first Cre-mediated recombination appeared at 16.5 dpc selectively at the heart. Like the endogenous cTnI gene, transgenic Cre expression showed a slow rise through fetal development that increased neonatally. Bitransgenic hearts, stained at 30 days of age, showed intense signals in ventricular and atrial myocytes while no recombination occurred in other tissues. The delayed onset of Cre activity in cTnI-Cre mice could provide a useful genetic tool to evaluate the function of loxP targeted cardiac genes without interference of recombination during early heart development.

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Language(s): eng - English
 Dates: 2007-04-262007-05-212007-05-212007-07-27
 Publication Status: Published in print
 Pages: 5
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: Biochemical and Biophysical Research Communications
  Other : Biochem. Biophys. Res. Commun.
Source Genre: Journal
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Publ. Info: Orlando, Fla. : Academic Press
Pages: - Volume / Issue: 359 (2) Sequence Number: - Start / End Page: 209 - 213 Identifier: ISSN: 0006-291X
CoNE: https://pure.mpg.de/cone/journals/resource/954922652205_1