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  Atrial-radiofrequency catheter ablation mediated targeting of mesenchymal stromal cells

Schweizer, P. A., Krause, U., Becker, R., Seckinger, A., Bauer, A., Hardt, C., et al. (2007). Atrial-radiofrequency catheter ablation mediated targeting of mesenchymal stromal cells. Stem Cells, 25(6), 1546-1551. doi:10.1634/stemcells.2006-0682.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-CB01-0 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-CB02-E
Genre: Journal Article
Alternative Title : Atrial-radiofrequency catheter ablation mediated targeting of mesenchymal stromal cells

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StemCells_25_ 2007_1546.pdf (Any fulltext), 649KB
 
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 Creators:
Schweizer, Patrick A.1, Author              
Krause, Ulf, Author
Becker, Ruediger, Author
Seckinger, Anja, Author
Bauer, Alexander, Author
Hardt, Cornelia, Author
Eckstein, Volker, Author
Ho, Anthony D., Author
Koenen, Michael1, 2, Author              
Katus, Hugo A., Author
Zehelein, Joerg2, Author              
Affiliations:
1Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497701              
2Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497704              

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Free keywords: Mesenchymal stromal cells; Catheter ablation; Pacemaker; Atrium
 Abstract: Sinus node dysfunction and high-degree heart block are the major causes for electronic pacemaker implantation. Recently, genetically modified mesenchymal stromal cells (MSCs, also known as "mesenchymal stem cells") were demonstrated to generate pacemaker function in vivo. However, experimental approaches typically use open thoracotomy for direct cell injection into the myocardium. Future clinical implementation, however, essentially requires development of more gentle methods to precisely and efficiently apply specified stem cells at specific cardiac locations. In a "proof of concept" study, we performed selective power-controlled radiofrequency catheter ablation (RFCA) with eight ablation pulses (30 W, 60 seconds each) to induce heat-mediated lesions at the auricles of the cardiac right atrium of four healthy foxhounds. The next day, allogeneic MSCs (4.3 x 10(5) cells per kilogram of body weight) labeled with superparamagnetic iron oxide particles (SPIOs) were infused intravenously. Hearts were explanted 8 days later. High numbers of SPIO-labeled cells were identified in areas surrounding the RFCA-induced lesions by Prussian blue staining. Antibody staining revealed SPIO-labeled cells being positive for the typical MSC surface antigen CD44. In contrast, low levels of calprotectin, an antigen found on monocytes and macrophages, indicated negligible infiltration of monocytes in MSC-positive areas. Thus, RFCA allows targeting of MSCs to the cardiac right atrium, adjacent to the sinoatrial node, providing an opportunity to rescue or generate pacemaker function without open thoracotomy and direct injection of MSCs. This method presents a new strategy for cardiac stem cell application leading to an efficient guidance of MSCs into the myocardium. Disclosure of potential conflicts of interest is found at the end of this article.

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Language(s): eng - English
 Dates: 2006-10-272007-01-262007-06-012007-06-01
 Publication Status: Published in print
 Pages: 6
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
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Title: Stem Cells
Source Genre: Journal
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Publ. Info: Dayton, Ohio : AlphaMed Press
Pages: - Volume / Issue: 25 (6) Sequence Number: - Start / End Page: 1546 - 1551 Identifier: ISSN: 1066-5099
CoNE: https://pure.mpg.de/cone/journals/resource/954925601690