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  Silencing and un-silencing of tetracycline-controlled genes in neurons

Zhu, P., Aller, M. I., Baron, U., Cambridge, S., Bausen, M., Herb, J., et al. (2007). Silencing and un-silencing of tetracycline-controlled genes in neurons. PLoS One, 2(6), 1-10. doi:10.1371/journal.pone.0000533.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-AE1B-F Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-AE1C-D
Genre: Journal Article
Alternative Title : Silencing and un-silencing of tetracycline-controlled genes in neurons

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 Creators:
Zhu, Peixin, Author
Aller, M. Isabel, Author
Baron, Udo, Author
Cambridge, Sidney , Author
Bausen, Melanie1, Author              
Herb, Jan2, Author              
Sawinski, Jürgen3, Author              
Cetin, Ali1, Author              
Osten, Pavel1, Author              
Nelson, Mark L., Author
Kügler, Sebastian, Author
Seeburg, Peter H.1, Author              
Sprengel, Rolf1, Author              
Hasan, Mazahir T.1, 3, Author              
Affiliations:
1Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497704              
2Max Planck Research Group Behavioural Neurophysiology (Andreas T. Schaefer), Max Planck Institute for Medical Research, Max Planck Society, ou_1497722              
3Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497699              

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 Abstract: To identify the underlying reason for the controversial performance of tetracycline (Tet)-controlled regulated gene expression in mammalian neurons, we investigated each of the three components that comprise the Tet inducible systems, namely tetracyclines as inducers, tetracycline-transactivator (tTA) and reverse tTA (rtTA), and tTA-responsive promoters (P(tets)). We have discovered that stably integrated P(tet) becomes functionally silenced in the majority of neurons when it is inactive during development. P(tet) silencing can be avoided when it is either not integrated in the genome or stably-integrated with basal activity. Moreover, long-term, high transactivator levels in neurons can often overcome integration-induced P(tet) gene silencing, possibly by inducing promoter accessibility.

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Language(s): eng - English
 Dates: 2007-03-132007-05-142007-06-20
 Publication Status: Published in print
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
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Title: PLoS One
Source Genre: Journal
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Publ. Info: San Francisco, CA : Public Library of Science
Pages: - Volume / Issue: 2 (6) Sequence Number: - Start / End Page: 1 - 10 Identifier: ISSN: 1932-6203
CoNE: https://pure.mpg.de/cone/journals/resource/1000000000277850