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  Adenosine-to-inosine RNA editing by ADAR1 is essential for normal murine erythropoiesis

Liddicoat, B. J., Hartner, J. C., Piskol, R., Ramaswami, G., Chalka, A. M., Kingsley, P. D., et al. (2016). Adenosine-to-inosine RNA editing by ADAR1 is essential for normal murine erythropoiesis. Experimental Hematology, 44(10), 947-963. doi:10.1016/j.exphem.2016.06.250.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-B282-6 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-B283-4
Genre: Journal Article

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 Creators:
Liddicoat, Brian J., Author
Hartner, Jochen C., Author
Piskol, Robert, Author
Ramaswami, Gokul, Author
Chalka, Alistair M., Author
Kingsley, Paul D., Author
Sankaran, Vijay G., Author
Wall, Meaghan, Author
Purton, Louise E., Author
Seeburg, Peter H.1, Author              
Palis, James, Author
Orkin, Stuart H., Author
Lu, Jun, Author
Li, Jin Billy, Author
Walkley, Carl R., Author
Affiliations:
1Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497704              

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 Abstract: Adenosine deaminases that act on RNA (ADARs) convert adenosine residues to inosine in doublestranded RNA. In vivo, ADAR1 is essential for the maintenance of hematopoietic stem/progenitors. Whether other hematopoietic cell types also require ADAR1 has not been assessed. Using erythroid- and myeloid-restricted deletion of Adar1, we demonstrate that ADAR1 is dispensable for myelopoiesis but is essential for normal erythropoiesis. Adar1-deficient erythroid cells display a profound activation of innate immune signaling and high levels of cell death. No changes in microRNAlevels were found inADAR1-deficient erythroid cells. Using an editing-deficient allele, we demonstrate thatRNA editing is the essential function ofADAR1 during erythropoiesis.Mapping of adenosine-to-inosine editing in purified erythroid cells identified clusters of hyperedited adenosines located in long 3’-untranslated regions of erythroid-specific transcripts and these are ADAR1-specific editing events. ADAR1-mediated RNA editing is essential for normal erythropoiesis.

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Language(s): eng - English
 Dates: 2016-06-012016-06-012016-07-012016-10-01
 Publication Status: Published in print
 Pages: 17
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.exphem.2016.06.250
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Title: Experimental Hematology
  Other : Exp. Hematol.
Source Genre: Journal
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Publ. Info: New York [etc.] : Elsevier
Pages: - Volume / Issue: 44 (10) Sequence Number: - Start / End Page: 947 - 963 Identifier: ISSN: 0301-472X
CoNE: https://pure.mpg.de/cone/journals/resource/954925510391