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  Rational design and applications of semisynthetic modular biosensors: SNIFITs and LUCIDs

Farrants, H., Hiblot, J., Griss, R., & Johnsson, K. (2017). Rational design and applications of semisynthetic modular biosensors: SNIFITs and LUCIDs. Methods in Molecular Biology, 1596, 101-117. doi:10.1007/978-1-4939-6940-1_7.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-B8A3-0 Version Permalink: http://hdl.handle.net/21.11116/0000-0001-4795-7
Genre: Journal Article

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MethMolBiol_1596_2017_101.pdf (Any fulltext), 594KB
 
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 Creators:
Farrants , Helen, Author
Hiblot, Julien1, Author              
Griss , Rudolf, Author
Johnsson, Kai1, Author              
Affiliations:
1Chemical Biology, Max Planck Institute for Medical Research, Max Planck Society, ou_2364732              

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Free keywords: Biosensors; CLIP-tag; Cell-surface biosensors; Intramolecular ligands; Polyproline linkers; Protein engineering; Protein switches; SNAP-tag; Self-labeling proteins; Therapeutic drug monitoring
 Abstract: Biosensors are used in many fields to measure the concentration of analytes, both in a cellular context and in human samples for medical care. Here, we outline the design of two types of modular biosensors: SNAP-tag-based indicators with a Fluorescent Intramolecular Tether (SNIFITs) and LUCiferase-based Indicators of Drugs (LUCIDs). These semisynthetic biosensors quantitatively measure analyte concentrations in vitro and on cell surfaces by an intramolecular competitive mechanism. We provide an overview of how to design and apply SNIFITs and LUCIDs.

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Language(s): eng - English
 Dates: 2017-05-152017
 Publication Status: Published in print
 Pages: 17
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: Methods in Molecular Biology
Source Genre: Series
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Publ. Info: New York : Springer
Pages: - Volume / Issue: 1596 Sequence Number: - Start / End Page: 101 - 117 Identifier: ISBN: 978-1-4939-6938-8
ISSN: 1064-3745