English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Colloid, adhesive and release properties of nanoparticular ternary complexes between cationic and anionic polysaccharides and basic proteins like bone morphogenetic protein BMP-2

Petzold, R., Vehlow, D., Urban, B., Grab, A., Cavalcanti-Adam, E. A., Alt, V., et al. (2017). Colloid, adhesive and release properties of nanoparticular ternary complexes between cationic and anionic polysaccharides and basic proteins like bone morphogenetic protein BMP-2. Colloids and Surfaces B: Biointerfaces, 151, 58-67. doi:10.1016/j.colsurfb.2016.11.029.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-C2AC-F Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002E-5DA5-C
Genre: Journal Article

Files

show Files
hide Files
:
Biointerfaces_151_2017_58.pdf (Any fulltext), 2MB
 
File Permalink:
-
Name:
Biointerfaces_151_2017_58.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Creators

show
hide
 Creators:
Petzold, R., Author
Vehlow, D., Author
Urban, B., Author
Grab, A.L., Author
Cavalcanti-Adam, E. A.1, 2, Author              
Alt, V., Author
Müller, M., Author
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany, ou_persistent22              

Content

show
hide
Free keywords: Bone healing; Bone substituting materials; Drug delivery system; Protein delivery; Biopolymer coating; Polyelectrolyte complex nanoparticles; Polysaccharides; Bone morphogenetic protein; FTIR spectroscopy; Circular dichroisma
 Abstract: Herein we describe an interfacial local drug delivery system for bone morphogenetic protein 2 (BMP-2) based on coatings of polyelectrolyte complex (PEC) nanoparticles (NP). The application horizon is the functionalization of bone substituting materials (BSM) used for the therapy of systemic bone diseases. Nanoparticular ternary complexes of cationic and anionic polysaccharides and BMP-2 or two further model proteins, respectively, were prepared in dependence of the molar mixing ratio, pH value and of the cationic polysaccharide. As further proteins chymotrypsin (CHY) and papain (PAP) were selected, which served as model proteins for BMP-2 due to similar isoelectric points and molecular weights. As charged polysaccharides ethylenediamine modified cellulose (EDAC) and trimethylammonium modified cellulose (PQ10) were combined with cellulose sulphatesulfate (CS). Mixing diluted cationic and anionic polysaccharide and protein solutions according to a slight either anionic or cationic excess charge colloidal ternary dispersions formed, which were cast onto germanium model substrates by water evaporation. Dynamic light scattering (DLS) demonstrated, that these dispersions were colloidally stable for at least one week. Fourier Transform Infrared (FTIR) showed, that the cast protein loaded PEC NP coatings were irreversibly adhesive at the model substrate in contact to HEPES buffer and solely CHY, PAP and BMP-2 were released within long-term time scale. Advantageously, out of the three proteins BMP-2 showed the smallest initial burst and the slowest release kinetics and around 25% of the initial BMP-2 content were released within 14days. Released BMP-2 showed significant activity in the myoblast cells indicating the ability to regulate the formation of new bone. Therefore, BMP-2 loaded PEC NP are suggested as novel promising tool for the functionalization of BSM used for the therapy of systemic bone diseases.

Details

show
hide
Language(s): eng - English
 Dates: 2016-10-192016-02-192016-11-232016-11-242017-03-01
 Publication Status: Published in print
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Colloids and Surfaces B: Biointerfaces
  Abbreviation : Colloids Surf., B
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Amsterdam : Elsevier
Pages: - Volume / Issue: 151 Sequence Number: - Start / End Page: 58 - 67 Identifier: ISSN: 0927-7765
CoNE: https://pure.mpg.de/cone/journals/resource/954926242488