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  Targeting of the Fun30 nucleosome remodeller by the Dpb11 scaffold facilitates cell cycle-regulated DNA end resection

Bantele, S. C. S., Ferreira, P., Gritenaite, D., Boos, D., & Pfander, B. (2017). Targeting of the Fun30 nucleosome remodeller by the Dpb11 scaffold facilitates cell cycle-regulated DNA end resection. eLife, 6: e21687. doi:10.7554/eLife.21687.

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 Creators:
Bantele, Susanne C. S.1, Author              
Ferreira, Pedro2, Author
Gritenaite, Dalia1, Author              
Boos, Dominik2, Author
Pfander, Boris1, Author              
Affiliations:
1Pfander, Boris / DNA Replication and Genome Integrity, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565165              
2external, ou_persistent22              

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Free keywords: DOUBLE-STRAND BREAK; REPAIR PATHWAY CHOICE; HOMOLOGOUS RECOMBINATION; SACCHAROMYCES-CEREVISIAE; DAMAGE CHECKPOINT; BUDDING YEAST; DISTINCT ROLES; PHOSPHORYLATION; REPLICATION; RECRUITMENTLife Sciences & Biomedicine - Other Topics;
 Abstract: DNA double strand breaks (DSBs) can be repaired by either recombination-based or direct ligation-based mechanisms. Pathway choice is made at the level of DNA end resection, a nucleolytic processing step, which primes DSBs for repair by recombination. Resection is thus under cell cycle control, but additionally regulated by chromatin and nucleosome remodellers. Here, we show that both layers of control converge in the regulation of resection by the evolutionarily conserved Fun30/SMARCAD1 remodeller. Budding yeast Fun30 and human SMARCAD1 are cell cycle-regulated by interaction with the DSB-localized scaffold protein Dpb11/TOPBP1, respectively. In yeast, this protein assembly additionally comprises the 9-1-1 damage sensor, is involved in localizing Fun30 to damaged chromatin, and thus is required for efficient long-range resection of DSBs. Notably, artificial targeting of Fun30 to DSBs is sufficient to bypass the cell cycle regulation of long-range resection, indicating that chromatin remodelling during resection is underlying DSB repair pathway choice.

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Language(s): eng - English
 Dates: 2017-01-12
 Publication Status: Published online
 Pages: 21
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000394261500001
DOI: 10.7554/eLife.21687
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Project name : Project Grant, PF794/3-1
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Funding organization : Deutsche Forschungsgemeinschaft

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Title: eLife
Source Genre: Journal
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Publ. Info: Cambridge : eLife Sciences Publications
Pages: - Volume / Issue: 6 Sequence Number: e21687 Start / End Page: - Identifier: Other: 2050-084X
CoNE: https://pure.mpg.de/cone/journals/resource/2050-084X