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  Structural basis of RNA polymerase I transcription initiation.

Engel, C., Gubbey, T., Neyer, S., Sainsbury, S., Oberthür, C., Bäjen, C., et al. (2017). Structural basis of RNA polymerase I transcription initiation. Cell, 169(1), 120-131. doi:10.1016/j.cell.2017.03.003.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-DD12-C Version Permalink: http://hdl.handle.net/21.11116/0000-0001-1F53-0
Genre: Journal Article

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 Creators:
Engel, C.1, Author              
Gubbey, T.1, Author              
Neyer, S.1, Author              
Sainsbury, S.1, Author              
Oberthür, C.1, Author              
Bäjen, C.1, Author              
Bernecky, C.1, Author              
Cramer, P.1, Author              
Affiliations:
1Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1863498              

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 Abstract: Transcription initiation at the ribosomal RNA promoter requires RNA polymerase (Pol) I and the initiation factors Rrn3 and core factor (CF). Here, we combine X-ray crystallography and cryo-electron microscopy (cryo-EM) to obtain a molecular model for basal Pol I initiation. The three-subunit CF binds upstream promoter DNA, docks to the Pol I-Rrn3 complex, and loads DNA into the expanded active center cleft of the polymerase. DNA unwinding between the Pol I protrusion and clamp domains enables cleft contraction, resulting in an active Pol I conformation and RNA synthesis. Comparison with the Pol II system suggests that promoter specificity relies on a distinct "bendability" and "meltability" of the promoter sequence that enables contacts between initiation factors, DNA, and polymerase.

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Language(s): eng - English
 Dates: 2017-03-232017-03-23
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.cell.2017.03.003
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Title: Cell
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Pages: - Volume / Issue: 169 (1) Sequence Number: - Start / End Page: 120 - 131 Identifier: -