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  Genome-wide analysis of RNA polymerase II termination at protein-coding genes.

Bäjen, C., Andreani, J., Torkler, P., Battaglia, S., Schwalb, B., Lidschreiber, M., et al. (2017). Genome-wide analysis of RNA polymerase II termination at protein-coding genes. Molecular Cell, 66(1), 38-49. doi:10.1016/j.molcel.2017.02.009.

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 Creators:
Bäjen, C.1, Author           
Andreani, J.2, Author           
Torkler, P.2, Author           
Battaglia, S.1, Author           
Schwalb, B.1, Author           
Lidschreiber, M.1, Author           
Maier, K. C.1, Author           
Boltendahl, A.1, Author           
Rus, P.1, Author           
Esslinger, S., Author
Söding, J.2, Author           
Cramer, P.1, Author           
Affiliations:
1Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1863498              
2Research Group of Computational Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1933286              

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 Abstract: At the end of protein-coding genes, RNA polymerase (Pol) II undergoes a concerted transition that involves 3′-processing of the pre-mRNA and transcription termination. Here, we present a genome-wide analysis of the 3′-transition in budding yeast. We find that the 3′-transition globally requires the Pol II elongation factor Spt5 and factors involved in the recognition of the polyadenylation (pA) site and in endonucleolytic RNA cleavage. Pol II release from DNA occurs in a narrow termination window downstream of the pA site and requires the “torpedo” exonuclease Rat1 (XRN2 in human). The Rat1-interacting factor Rai1 contributes to RNA degradation downstream of the pA site. Defects in the 3′-transition can result in increased transcription at downstream genes.

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Language(s): eng - English
 Dates: 2017-03-162017-04-06
 Publication Status: Published in print
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 Rev. Type: Peer
 Identifiers: DOI: 10.1016/j.molcel.2017.02.009
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Title: Molecular Cell
Source Genre: Journal
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Pages: - Volume / Issue: 66 (1) Sequence Number: - Start / End Page: 38 - 49 Identifier: -