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  Transcriptional and Chromatin Dynamics of Muscle Regeneration after Severe Trauma

Aguilar, C. A., Pop, R., Shcherbina, A., Watts, A., Matheny Jr., R. W., Cacchiarelli, D., et al. (2016). Transcriptional and Chromatin Dynamics of Muscle Regeneration after Severe Trauma. Stem Cell Reports, 7(5), 983-997. doi:10.1016/j.stemcr.2016.09.009.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-DEE3-E Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-DEE4-C
Genre: Journal Article

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© 2016 The Author(s). Published by Elsevier Inc.

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Aguilar, Carlos A. , Author
Pop, Ramona, Author
Shcherbina, Anna, Author
Watts, Alain, Author
Matheny Jr., Ronald W., Author
Cacchiarelli, Davide , Author
Han, Woojin M. , Author
Shin, Eunjung , Author
Nakhai, Shadi A. , Author
Jang, Young C. , Author
Carrigan, Christopher T. , Author
Gifford, Casey A. , Author
Kottke, Melissa A., Author
Cesana, Marcella , Author
Lee, Jackson, Author
Urso, Maria L. , Author
Meissner, Alexander1, 2, Author              
Affiliations:
1Dept. of Genome Regulation (Head: Alexander Meissner), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_2379694              
2Department of Stem Cell and Regenerative Biology, Harvard Stem Cell Institute, Harvard University, Cambridge, MA 02138, USA, ou_persistent22              

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 Abstract: Following injury, adult skeletal muscle undergoes a well-coordinated sequence of molecular and physiological events to promote repair and regeneration. However, a thorough understanding of the in vivo epigenomic and transcriptional mechanisms that control these reparative events is lacking. To address this, we monitored the in vivo dynamics of three histone modifications and coding and noncoding RNA expression throughout the regenerative process in a mouse model of traumatic muscle injury. We first illustrate how both coding and noncoding RNAs in tissues and sorted satellite cells are modified and regulated during various stages after trauma. Next, we use chromatin immunoprecipitation followed by sequencing to evaluate the chromatin state of cis-regulatory elements (promoters and enhancers) and view how these elements evolve and influence various muscle repair and regeneration transcriptional programs. These results provide a comprehensive view of the central factors that regulate muscle regeneration and underscore the multiple levels through which both transcriptional and epigenetic patterns are regulated to enact appropriate repair and regeneration.

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Language(s): eng - English
 Dates: 2016-10-202016-11-08
 Publication Status: Published in print
 Pages: 15
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: DOI: 10.1016/j.stemcr.2016.09.009
 Degree: -

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Title: Stem Cell Reports
Source Genre: Journal
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Publ. Info: Elsevier B. V.
Pages: - Volume / Issue: 7 (5) Sequence Number: - Start / End Page: 983 - 997 Identifier: -