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  Transcriptional signature induced by a metastasis-promoting c-Src mutant in a human breast cell line

Broecker, F., Hardt, C., Herwig, R., Timmermann, B., Kerick, M., Wunderlich, A., et al. (2016). Transcriptional signature induced by a metastasis-promoting c-Src mutant in a human breast cell line. The FEBS Journal, 283(9), 1669-1688. doi:10.1111/febs.13694.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-46DD-9 Version Permalink: http://hdl.handle.net/21.11116/0000-0000-F9EC-E
Genre: Journal Article

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© 2016 Federation of European Biochemical Societies
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 Creators:
Broecker, F.1, Author              
Hardt, C.2, Author              
Herwig, R.2, Author              
Timmermann, B.3, Author              
Kerick, M., Author
Wunderlich, A., Author
Schweiger, M. R., Author
Borsig, L., Author
Heikenwalder, M., Author
Lehrach, H.4, Author              
Moelling, K.1, Author              
Affiliations:
1Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              
2Bioinformatics (Ralf Herwig), Dept. of Computational Molecular Biology (Head: Martin Vingron), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_2385701              
3Sequencing (Head: Bernd Timmermann), Scientific Service (Head: Christoph Krukenkamp), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479670              
4Emeritus Group of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_2385697              

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Free keywords: PDZ domain c-Src metastasis oncogene transcriptome
 Abstract: Deletions at the C-terminus of the proto-oncogene protein c-Src kinase are found in the viral oncogene protein v-Src as well as in some advanced human colon cancers. They are associated with increased kinase activity and cellular invasiveness. Here, we analyzed the mRNA expression signature of a constitutively active C-terminal mutant of c-Src, c-Src(mt), in comparison with its wild-type protein, c-Src(wt), in the human non-transformed breast epithelial cell line MCF-10A. We demonstrated previously that the mutant altered migratory and metastatic properties. Genome-wide transcriptome analysis revealed that c-Src(mt) de-regulated the expression levels of approximately 430 mRNAs whose gene products are mainly involved in the cellular processes of migration and adhesion, apoptosis and protein synthesis. 82.9% of these genes have previously been linked to cellular migration, while the others play roles in RNA transport and splicing processes, for instance. Consistent with the transcriptome data, cells expressing c-Src(mt), but not those expressing c-Src(wt), showed the capacity to metastasize into the lungs of mice in vivo. The mRNA expression profile of c-Src(mt)-expressing cells shows significant overlap with that of various primary human tumor samples, possibly reflecting elevated Src activity in some cancerous cells. Expression of c-Src(mt) led to elevated migratory potential. We used this model system to analyze the transcriptional changes associated with an invasive cellular phenotype. These genes and pathways de-regulated by c-Src(mt) may provide suitable biomarkers or targets of therapeutic approaches for metastatic cells. DATABASE: This project was submitted to the National Center for Biotechnology Information BioProject under ID PRJNA288540. The Illumina RNA-Seq reads are available in the National Center for Biotechnology Information Sequence Read Archive under study ID SRP060008 with accession numbers SRS977414 for MCF-10A cells, SRS977717 for mock cells, SRS978053 for c-Src(wt) cells and SRS978046 for c-Src(mt) cells.

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Language(s): eng - English
 Dates: 2016-03-252016-05
 Publication Status: Published in print
 Pages: 20
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: PMID: 26919036
DOI: 10.1111/febs.13694
ISSN: 1742-4658 (Electronic)1742-464X (Print)
 Degree: -

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Title: The FEBS Journal
  Other : The Federation if European Biochemical Societies Journal
Source Genre: Journal
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Publ. Info: Wiley-Blackwell
Pages: - Volume / Issue: 283 (9) Sequence Number: - Start / End Page: 1669 - 1688 Identifier: ISSN: 1742-464X
CoNE: /journals/resource/954925398485