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  Genome-wide Chromatin Profiling of Legionella pneumophila-Infected Human Macrophages Reveals Activation of the Probacterial Host Factor TNFAIP2

Du Bois, I., Marsico, A., Bertrams, W., Schweiger, M. R., Caffrey, B., Sittka-Stark, A., et al. (2016). Genome-wide Chromatin Profiling of Legionella pneumophila-Infected Human Macrophages Reveals Activation of the Probacterial Host Factor TNFAIP2. The Journal of Infectious Diseases, 214(3), 454-463. doi:10.1093/infdis/jiw171.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-46E6-4 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-46E7-2
Genre: Journal Article

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Du Bois.pdf (Publisher version), 706KB
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 Creators:
Du Bois, I., Author
Marsico, A.1, Author              
Bertrams, W., Author
Schweiger, M. R., Author
Caffrey, B.1, Author              
Sittka-Stark, A., Author
Eberhardt, M., Author
Vera, J., Author
Vingron, M.2, Author              
Schmeck, B. T., Author
Affiliations:
1RNA Bioinformatics (Annalisa Marsico), Independent Junior Research Groups (OWL), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_2117285              
2Gene regulation (Martin Vingron), Dept. of Computational Molecular Biology (Head: Martin Vingron), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479639              

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Free keywords: A549 Legionella pneumophila Tnfaip2 macrophage
 Abstract: BACKGROUND: Legionella pneumophila is a causative agent of severe pneumonia. Infection leads to a broad host cell response, as evident, for example, on the transcriptional level. Chromatin modifications, which control gene expression, play a central role in the transcriptional response to L. pneumophila METHODS: We infected human-blood-derived macrophages (BDMs) with L. pneumophila and used chromatin immunoprecipitation followed by sequencing to screen for gene promoters with the activating histone 4 acetylation mark. RESULTS: We found the promoter of tumor necrosis factor alpha-induced protein 2 (TNFAIP2) to be acetylated at histone H4. This factor has not been characterized in the pathology of L. pneumophila TNFAIP2 messenger RNA and protein were upregulated in response to L. pneumophila infection of human-BDMs and human alveolar epithelial (A549) cells. We showed that L. pneumophila-induced TNFAIP2 expression is dependent on the NF-kappaB transcription factor. Importantly, knock down of TNFAIP2 led to reduced intracellular replication of L. pneumophila Corby in A549 cells. CONCLUSIONS: Taken together, genome-wide chromatin analysis of L. pneumophila-infected macrophages demonstrated induction of TNFAIP2, a NF-kappaB-dependent factor relevant for bacterial replication.

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Language(s): eng - English
 Dates: 2016-04-292016-08
 Publication Status: Published in print
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: PMID: 27130431
DOI: 10.1093/infdis/jiw171
ISSN: 1537-6613 (Electronic)0022-1899 (Print)
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Title: The Journal of Infectious Diseases
Source Genre: Journal
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Publ. Info: Chicago, Ill. : Published by the University of Chicago Press for the Infectious Diseases Society of America
Pages: - Volume / Issue: 214 (3) Sequence Number: - Start / End Page: 454 - 463 Identifier: ISSN: 0022-1899
CoNE: https://pure.mpg.de/cone/journals/resource/954925414917_1