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  Evolution of DNA-Binding Sites of a Floral Master Regulatory Transcription Factor

Muino, J. M., de Bruijn, S., Pajoro, A., Geuten, K., Vingron, M., Angenent, G. C., et al. (2016). Evolution of DNA-Binding Sites of a Floral Master Regulatory Transcription Factor. Molecular Biology and Evolution, 33(1), 185-200. doi:10.1093/molbev/msv210.

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Muiño.pdf (Publisher version), 935KB
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 Creators:
Muino, J. M.1, Author              
de Bruijn, S., Author
Pajoro, A., Author
Geuten, K., Author
Vingron, M.2, Author              
Angenent, G. C., Author
Kaufmann, K., Author
Affiliations:
1Dept. of Computational Molecular Biology (Head: Martin Vingron), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433547              
2Gene regulation (Martin Vingron), Dept. of Computational Molecular Biology (Head: Martin Vingron), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479639              

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Free keywords: MADS-domain transcription factor cis-regulatory evolution plant development
 Abstract: Flower development is controlled by the action of key regulatory transcription factors of the MADS-domain family. The function of these factors appears to be highly conserved among species based on mutant phenotypes. However, the conservation of their downstream processes is much less well understood, mostly because the evolutionary turnover and variation of their DNA-binding sites (BSs) among plant species have not yet been experimentally determined. Here, we performed comparative ChIP (chromatin immunoprecipitation)-seq experiments of the MADS-domain transcription factor SEPALLATA3 (SEP3) in two closely related Arabidopsis species: Arabidopsis thaliana and A. lyrata which have very similar floral organ morphology. We found that BS conservation is associated with DNA sequence conservation, the presence of the CArG-box BS motif and on the relative position of the BS to its potential target gene. Differences in genome size and structure can explain that SEP3 BSs in A. lyrata can be located more distantly to their potential target genes than their counterparts in A. thaliana. In A. lyrata, we identified transposition as a mechanism to generate novel SEP3 binding locations in the genome. Comparative gene expression analysis shows that the loss/gain of BSs is associated with a change in gene expression. In summary, this study investigates the evolutionary dynamics of DNA BSs of a floral key-regulatory transcription factor and explores factors affecting this phenomenon.

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Language(s): eng - English
 Dates: 2016-01
 Publication Status: Published in print
 Pages: 16
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: PMID: 26429922
DOI: 10.1093/molbev/msv210
ISSN: 1537-1719 (Electronic)0737-4038 (Print)
 Degree: -

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Title: Molecular Biology and Evolution
  Other : Mol. Biol. Evol.
Source Genre: Journal
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Publ. Info: Oxford : Oxford University Press
Pages: - Volume / Issue: 33 (1) Sequence Number: - Start / End Page: 185 - 200 Identifier: ISSN: 0737-4038
CoNE: https://pure.mpg.de/cone/journals/resource/954925536119