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  Low-dose fixed-target serial synchrotron crystallography

Owen, R. L., Axford, D., Sherrell, D. A., Kuo, A., Ernst, O. P., Schulz, E.-C., et al. (2017). Low-dose fixed-target serial synchrotron crystallography. Acta Crystallographica Section D: Structural Biology, 73, 373-378. doi:10.1107/S2059798317002996.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-E821-F Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-E822-D
Genre: Journal Article

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Low-dose fixed-target serial synchrotron crystallography.pdf (Publisher version), 2MB
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2017-04-01
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Owen et. al.

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 Creators:
Owen, Robin L.1, Author
Axford, Danny1, Author
Sherrell, Darren A.1, Author
Kuo, Anling2, Author
Ernst, Oliver P.2, 3, Author
Schulz, Eike-Christian4, Author              
Miller, R. J. Dwayne4, 5, Author              
Müller-Werkmeister, Henrike4, Author              
Affiliations:
1Diamond Light Source, Harwell Science and Innovation Campus, Didcot OX11 0DE, England, ou_persistent22              
2Department of Biochemistry, University of Toronto, King's College Circle , Toronto, Ontario M5S 1A8, Canada, , ou_persistent22              
3Department of Molecular Genetics, University of Toronto, King's College Circle, Toronto, Ontario M5S 1A8, Canada, ou_persistent22              
4Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society, ou_1938288              
5Departments of Chemistry and Physics, University of Toronto, King's College Circle, Toronto, Ontario M5S 1A8, Canada, ou_persistent22              

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Free keywords: macromolecular crystallography serial synchrotron crystallography fixed target room-temperature crystallography crystallography on a chip low dose
 Abstract: The development of serial crystallography has been driven by the sample requirements imposed by X-ray free-electron lasers. Serial techniques are now being exploited at synchrotrons. Using a fixed-target approach to high-throughput serial sampling, it is demonstrated that high-quality data can be collected from myoglobin crystals, allowing room-temperature, low-dose structure determination. The combination of fixed-target arrays and a fast, accurate translation system allows high-throughput serial data collection at high hit rates and with low sample consumption.

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Language(s): eng - English
 Dates: 2016-10-202017-02-222017-04-01
 Publication Status: Published online
 Pages: 6
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1107/S2059798317002996
 Degree: -

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Title: Acta Crystallographica Section D: Structural Biology
  Abbreviation : Acta Cryst. D
Source Genre: Journal
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Publ. Info: Chester, England : International Union of Crystallography
Pages: 6 Volume / Issue: 73 Sequence Number: - Start / End Page: 373 - 378 Identifier: ISSN: 2059-7983
CoNE: /journals/resource/2059-7983