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  Hydroxylated fluorescent dyes for live-cell labeling: Synthesis, spectra and super-resolution STED.

Butkevich, A., Belov, V. N., Kolmakov, K., Sokolov, V. V., Shojaei, H., Sidenstein, S., et al. (2017). Hydroxylated fluorescent dyes for live-cell labeling: Synthesis, spectra and super-resolution STED. Chemistry. A European Journal, 23(50), 12114-12119. doi:10.1002/chem.201701216.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-E95B-9 Version Permalink: http://hdl.handle.net/21.11116/0000-0001-1926-9
Genre: Journal Article

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 Creators:
Butkevich, A.1, Author              
Belov, V. N.1, Author              
Kolmakov, K., Author
Sokolov, V. V., Author
Shojaei, H.1, Author              
Sidenstein, S.1, Author              
Kamin, D.1, Author              
Mattias, J., Author
Vlijm, R., Author
Engelhardt, J., Author
Hell, S. W.1, Author              
Affiliations:
1Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society, ou_578627              

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Free keywords: fluorescent dyes, rhodamines, germanorhodamines, optical microscopy, living cells
 Abstract: Hydroxylated rhodamines, carbopyronines, silico- and germanorhodamines with absorption maxima in the range of 530-640 nm were prepared and applied in specific labeling of living cells. The direct and high-yielding entry to germa- and silaxanthones tolerates the presence of protected heteroatoms and may be considered for the syntheses of various sila- and germafluoresceins, as well as -rhodols. Application in stimulated emission depletion (STED) fluorescence microscopy revealed a resolution of 50-75 nm in one- and two-color imaging of vimentin-HaloTag fused protein and native tubulin. The established structure-property relationships allow prediction of the spectral properties and the positions of spirolactone/zwitterion equilibria for the new analogs of rhodamines, carbo-, silico- and germanorhodamines using simple additive schemes.

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Language(s): eng - English
 Dates: 2017-05-022017-09-07
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1002/chem.201701216
 Degree: -

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Title: Chemistry. A European Journal
Source Genre: Journal
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Pages: - Volume / Issue: 23 (50) Sequence Number: - Start / End Page: 12114 - 12119 Identifier: -