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  Mapping the secretome of human chondrogenic progenitor cells with mass spectrometry.

Batschkus, S., Atanassov, I., Lenz, C., Meyer-Marcotty, P., Cingöz, G., Kirschneck, C., et al. (2017). Mapping the secretome of human chondrogenic progenitor cells with mass spectrometry. Annals of Anatomy, 212, 4-10. doi:10.1016/j.aanat.2017.03.003.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-EA41-A Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-092A-1
Genre: Journal Article

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2418537.pdf (Publisher version), 2MB
 
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 Creators:
Batschkus, S., Author
Atanassov, I.1, Author              
Lenz, C.1, Author              
Meyer-Marcotty, P., Author
Cingöz, G., Author
Kirschneck, C., Author
Urlaub, H.1, Author              
Miosge, N., Author
Affiliations:
1Research Group of Bioanalytical Mass Spectrometry, MPI for Biophysical Chemistry, Max Planck Society, ou_578613              

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Free keywords: Osteoarthritis; chondrogenic progenitor cells; mass spectrometry; secretome; tissue engineering
 Abstract: Tissue engineering offers promising perspectives in the therapy of osteoarthritis. In the context of cell-based therapy, chondrogenic progenitor cells (CPCs) may be used to regenerate defects in cartilage tissue. An in-depth characterization of the secretome of CPCs is a prerequisite to this approach. In this study, a method was developed for the qualitative and quantitative analysis of the secretome of undifferentiated and differentiated CPCs. Secreted proteins from cells grown in two-dimensional as well as three-dimensional alginate cultures were extracted and analyzed by liquid chromatography/tandem mass spectrometry (LC-MS/MS). Quantitation was achieved by internal standardization using stable isotope-labeled amino acids in cell culture (SILAC). Qualitative analysis of CPC secretomes revealed ECM-components, signal proteins and growth factors most of which were also found in healthy cartilage. A quantitative comparison revealed significantly upregulated proteins with regenerative potential during differentiation, while proteins involved in catabolic metabolism were significantly downregulated. The development of methods for qualitative and quantitative analysis of the secretome of CPCs by mass spectrometry provides a foundation for the investigation of progenitor or stem cells from other sources.

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Language(s): eng - English
 Dates: 2017-03-292017-07
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.aanat.2017.03.003
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Title: Annals of Anatomy
Source Genre: Journal
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Pages: - Volume / Issue: 212 Sequence Number: - Start / End Page: 4 - 10 Identifier: -