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  Architecture of a transcribing-translating expressome.

Kohler, R., Mooney, R. A., Mills, D. J., Landick, R., & Cramer, P. (2017). Architecture of a transcribing-translating expressome. Science, 356(6334), 194-197. doi:10.1126/science.aal3059.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-080F-7 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002E-77CD-E
Genre: Journal Article

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 Creators:
Kohler, R.1, Author              
Mooney, R. A., Author
Mills, D. J., Author
Landick, R., Author
Cramer, P.1, Author              
Affiliations:
1Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1863498              

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 Abstract: DNA transcription is functionally coupled to messenger RNA (mRNA) translation in bacteria, but how this is achieved remains unclear. Here we show that RNA polymerase (RNAP) and the ribosome of Escherichia coli can form a defined transcribing and translating “expressome” complex. The cryo–electron microscopic structure of the expressome reveals continuous protection of ~30 nucleotides of mRNA extending from the RNAP active center to the ribosome decoding center. The RNAP-ribosome interface includes the RNAP subunit α carboxyl-terminal domain, which is required for RNAP-ribosome interaction in vitro and for pronounced cell growth defects upon translation inhibition in vivo, consistent with its function in transcription-translation coupling. The expressome structure can only form during transcription elongation and explains how translation can prevent transcriptional pausing, backtracking, and termination.

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Language(s): eng - English
 Dates: 2017-04-142017-04-14
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1126/science.aal3059
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Title: Science
Source Genre: Journal
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Pages: - Volume / Issue: 356 (6334) Sequence Number: - Start / End Page: 194 - 197 Identifier: -