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  A Wnt5 activity asymmetry and intercellular signaling via PCP proteins polarize node cells for left-right symmetry breaking.

Minegishi, K., Hashimoto, M., Ajima, R., Takaoka, K., Shinohara, K., Ikawa, Y., et al. (2017). A Wnt5 activity asymmetry and intercellular signaling via PCP proteins polarize node cells for left-right symmetry breaking. Developmental Cell, 40(5), 439-452. doi:10.1016/j.devcel.2017.02.010.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-08A6-4 Version Permalink: http://hdl.handle.net/21.11116/0000-0001-3B77-8
Genre: Journal Article

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 Creators:
Minegishi, K., Author
Hashimoto, M., Author
Ajima, R., Author
Takaoka, K.1, Author              
Shinohara, K., Author
Ikawa, Y., Author
Nishimura, H., Author
McMahon, A. P., Author
Willert, K., Author
Okada, Y., Author
Affiliations:
1Department of Meiosis, MPI for Biophysical Chemistry, Max Planck Society, ou_2205654              

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Free keywords: planar cell polarity; breaking of left-right symmetry; Wnt; Sfrp; cilia; basal body
 Abstract: Polarization of node cells along the anterior-posterior axis of mouse embryos is responsible for left-right symmetry breaking. How node cells become polarized has remained unknown, however. Wnt5a and Wnt5b are expressed posteriorly relative to the node, whereas genes for Sfrp inhibitors of Wnt signaling are expressed anteriorly. Here we show that polarization of node cells is impaired in Wnt5a–/–Wnt5b–/– and Sfrp mutant embryos, and also in the presence of a uniform distribution of Wnt5a or Sfrp1, suggesting that Wnt5 and Sfrp proteins act as instructive signals in this process. The absence of planar cell polarity (PCP) core proteins Prickle1 and Prickle2 in individual cells or local forced expression of Wnt5a perturbed polarization of neighboring wild-type cells. Our results suggest that opposing gradients of Wnt5a and Wnt5b and of their Sfrp inhibitors, together with intercellular signaling via PCP proteins, polarize node cells along the anterior-posterior axis for breaking of left-right symmetry.

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Language(s): eng - English
 Dates: 2017-03-132017-03
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.devcel.2017.02.010
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Title: Developmental Cell
Source Genre: Journal
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Pages: - Volume / Issue: 40 (5) Sequence Number: - Start / End Page: 439 - 452 Identifier: -