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  Automated glycan assembly using the Glyconeer 2.1 synthesizer

Hahm, H. S., Schlegel, M. K., Hurevich, M., Eller, S., Schuhmacher, F., Hofmann, J., et al. (2017). Automated glycan assembly using the Glyconeer 2.1 synthesizer. Proceedings of the National Academy of Sciences of the United States of America, 114(17), E3385-E3389. doi:10.1073/pnas.1700141114.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-2F33-A Version Permalink: http://hdl.handle.net/21.11116/0000-0006-5910-4
Genre: Journal Article

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 Creators:
Hahm, Heung Sik1, Author              
Schlegel, Mark K.1, Author              
Hurevich, Mattan2, Author              
Eller, Steffen2, Author              
Schuhmacher, Frank1, Author              
Hofmann, Johanna, Author
Pagel, Kevin, Author
Seeberger, Peter H.1, Author              
Affiliations:
1Peter H. Seeberger - Automated Systems, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863306              
2Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863286              

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 Abstract: Reliable and rapid access to defined biopolymers by automated DNA and peptide synthesis has fundamentally altered biological research and medical practice. Similarly, the procurement of defined glycans is key to establishing structure–activity relationships and thereby progress in the glycosciences. Here, we describe the rapid assembly of oligosaccharides using the commercially available Glyconeer 2.1 automated glycan synthesizer, monosaccharide building blocks, and a linker-functionalized polystyrene solid support. Purification and quality-control protocols for the oligosaccharide products have been standardized. Synthetic glycans prepared in this way are useful reagents as the basis for glycan arrays, diagnostics, and carbohydrate-based vaccines.

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 Dates: 2017-042017
 Publication Status: Published in print
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 Identifiers: DOI: 10.1073/pnas.1700141114
PMC: PMC5410834
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Project name : Autoheparin
Grant ID : 227975
Funding program : Funding Programme 7 (FP7)
Funding organization : European Commission (EC)

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Title: Proceedings of the National Academy of Sciences of the United States of America
  Other : Proc. Acad. Sci. USA
  Other : Proc. Acad. Sci. U.S.A.
  Abbreviation : PNAS
Source Genre: Journal
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Publ. Info: Washington, D.C. : National Academy of Sciences
Pages: - Volume / Issue: 114 (17) Sequence Number: - Start / End Page: E3385 - E3389 Identifier: ISSN: 0027-8424