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Abstract:
Although many established tools for cytokinin (CK) pathway manipulations are well suitable for the
analysis
of molecular interactions, their use on a whole plant scale is often limited by the induction of
severe developmental defects. To circumvent this problem, different methods were developed that allow
for a more precise manipulation of the CK pathway. Here we present one of these systems, the pOp6/
LhGR system for chemically inducible gene expression. This system allows regulation on a spatial, temporal,
and quantitative scale and therefore provides a superior tool for analyzing the role of CKs in the interactions
of plants with their environment. The pOp6/LhGR system was tested for RNAi-mediated gene
silencing and heterologous gene expression and was successfully used for CK pathway manipulations in
different model organisms (Arabidopsis thaliana, Nicotiana tabaccum, Nicotiana attenuata, Citrus sinensis
× C. trifoliate). Here we describe specific aspects of the screening procedure and present an experimental
setup that can not only be used in the laboratory but is also applicable under field conditions.
