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  Hierarchical RNA Processing Is Required for Mitochondrial Ribosome Assembly.

Rackham, O., Busch, J. D., Matic, S., Siira, S. J., Kuznetsova, I., Atanassov, I., et al. (2016). Hierarchical RNA Processing Is Required for Mitochondrial Ribosome Assembly. Cell reports, 16(7), 1874-1890. doi:10.1016/j.celrep.2016.07.031.

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 Creators:
Rackham, Oliver1, Author
Busch, Jakob D1, Author
Matic, Stanka1, Author
Siira, Stefan J1, Author
Kuznetsova, Irina1, Author
Atanassov, Ilian1, Author
Ermer, Judith A1, Author
Shearwood, Anne-Marie J1, Author
Richman, Tara R1, Author
Stewart, James B1, Author
Mourier, Arnaud1, Author
Milenkovic, Dusanka1, Author
Larsson, Nils-Göran1, Author
Filipovska, Aleksandra1, Author
Affiliations:
1Max Planck Institute for Biology of Ageing, Max Planck Society, Joseph-Stelzmann-Str. 9b, D-50931 Cologne, DE, ou_1942284              

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 Abstract: The regulation of mitochondrial RNA processing and its importance for ribosome biogenesis and energy metabolism are not clear. We generated conditional knockout mice of the endoribonuclease component of the RNase P complex, MRPP3, and report that it is essential for life and that heart and skeletal-muscle-specific knockout leads to severe cardiomyopathy, indicating that its activity is non-redundant. Transcriptome-wide parallel analyses of RNA ends (PARE) and RNA-seq enabled us to identify that in vivo 5' tRNA cleavage precedes 3' tRNA processing, and this is required for the correct biogenesis of the mitochondrial ribosomal subunits. We identify that mitoribosomal biogenesis proceeds co-transcriptionally because large mitoribosomal proteins can form a subcomplex on an unprocessed RNA containing the 16S rRNA. Taken together, our data show that RNA processing links transcription to translation via assembly of the mitoribosome.

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Language(s): eng - English
 Dates: 2016
 Publication Status: Published in print
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 Rev. Type: -
 Identifiers: Other: 27498866
DOI: 10.1016/j.celrep.2016.07.031
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Title: Cell reports
Source Genre: Journal
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Pages: - Volume / Issue: 16 (7) Sequence Number: - Start / End Page: 1874 - 1890 Identifier: -