English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Properties of cardiolipin and functional implications for cytochrome oxidase activity.

Marsh, D., & Powell, G. L. (1988). Properties of cardiolipin and functional implications for cytochrome oxidase activity. Bioelectrochemistry and Bioenergetics, 20(1-3), 73-82. doi:10.1016/S0302-4598(98)80006-8.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-2BC8-B Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-2BCB-5
Genre: Journal Article

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Marsh, D.1, Author              
Powell, G. L., Author
Affiliations:
1Department of Spectroscopy and Photochemical Kinetics, MPI for biophysical chemistry, Max Planck Society, ou_578624              

Content

show
hide
Free keywords: -
 Abstract: Cardiolipin derivatives with varying numbers of acyl chains ranging from two to five are found to display a wide variety of polymorphic forms in aqueous dispersion, varying from micelles through bilayers to inverted hexagonal phases. Dilyso cardiolipin and cardiolipin itself are found to change their polymorphic form at high salt concentrations ([NaCl] > 1.5 M), changing from micelle to bilayer or from bilayer to inverted hexagonal phase, respectively. Spin-labelled cardiolipin analogues are found to exhibit a preferential interaction with reconstituted cytochrome oxidase in a dimyristoyl phosphatidylcholine environment. Varying the number of acyl chains in the cardiolipin molecule from three to five is found to have only a small effect on the selectivity of the lipid-protein interaction. Removal of the cardiolipin charge by methylation of the phosphate groups, or screening of the electrostatic interactions by high salt concentrations ([NaCl] > 1.5 M), is found to reduce, but not to abolish, the selectivity. Cardiolipin derivatives are found to enhance considerably the activity of cytochrome oxidase reconstituted in dimyristoyl phosphatidylcholine, at all substrate concentrations. Possible mechanisms for activation by cardiolipin are discussed in the light of the physical measurements.

Details

show
hide
Language(s): eng - English
 Dates: 1988-12
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1016/S0302-4598(98)80006-8
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Bioelectrochemistry and Bioenergetics
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 20 (1-3) Sequence Number: - Start / End Page: 73 - 82 Identifier: -