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  1-O-methyl-rac-glycerol: A new agent for the cryopreservation of mononuclear cells.

Schuff-Werner, P., Müller, U., Unger, C., & Eibl, H. (1988). 1-O-methyl-rac-glycerol: A new agent for the cryopreservation of mononuclear cells. Cryobiology, 25(6), 487-494. doi:10.1016/0011-2240(88)90294-5.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-2BEA-0 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-2BED-A
Genre: Journal Article

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Schuff-Werner, P., Author
Müller, U., Author
Unger, C., Author
Eibl, H.1, Author              
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1Department of Membrane Biophysics, MPI for biophysical chemistry, Max Planck Society, ou_578579              

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 Abstract: 1-O-methyl-rac-glycerol (1-O-MG), also known as 3-methyoxy-1,2-propanediol is a lipophilic derivative of glycerol, and has been studied as a new cryoprotective agent (CPA) for mononuclear blood cells (MNC), a well-established experimental model in cryopreservation. The chemical modification of the glycerol molecule results in improved cryobiological properties, such as membrane permeability, thus allowing easier handling in the freeze/thaw process. The optimum preincubation period for MNC and 1-O-MG before freezing is 5 min at 4 °C, resulting in 86% recovery of viable cells, whereas optimal recovery of glycerol-frozen MNC is only guaranteed after 30 min of preincubation at room temperature (74% viable recovery). The optimal concentration of 1-O-MG is 10% (). Although this new agent offers no improvement in cryoprotective properties over dimethyl sulfoxide (Me2SO) there may be possible pharmacological advantages when used in humans. It is, however, obviously superior to glycerol with regard to its permeation kinetics. 1-O-MG might therefore also be of interest in the cryoprotection of other hematopoetic cells and biological tissues.

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Language(s): eng - English
 Dates: 1988-12
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1016/0011-2240(88)90294-5
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Title: Cryobiology
Source Genre: Journal
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Pages: - Volume / Issue: 25 (6) Sequence Number: - Start / End Page: 487 - 494 Identifier: -