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  Universal Super-Resolution Multiplexing by DNA Exchange

Schueder, F., Strauss, M. T., Hoerl, D., Schnitzbauer, J., Schlichthaerle, T., Strauss, S., et al. (2017). Universal Super-Resolution Multiplexing by DNA Exchange. Angewandte Chemie International Edition, 56(14), 4052-4055. doi:10.1002/anie.201611729.

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 Creators:
Schueder, Florian1, Author              
Strauss, Maximilian T.1, Author              
Hoerl, David2, Author
Schnitzbauer, Joerg1, Author              
Schlichthaerle, Thomas1, Author              
Strauss, Sebastian1, Author              
Yin, Peng2, Author
Harz, Hartmann2, Author
Leonhardt, Heinrich2, Author
Jungmann, Ralf1, Author              
Affiliations:
1Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society, ou_2149679              
2external, ou_persistent22              

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Free keywords: TRANSIENT BINDING; MICROSCOPY; FORMAMIDE; PROTEINS; ORIGAMI; PROBES; LIMITChemistry; DNA nanotechnology; dSTORM; multiplexing; SIM; STED;
 Abstract: Super-resolution microscopy allows optical imaging below the classical diffraction limit of light with currently up to 20 x higher spatial resolution. However, the detection of multiple targets (multiplexing) is still hard to implement and time-consuming to conduct. Here, we report a straightforward sequential multiplexing approach based on the fast exchange of DNA probes which enables efficient and rapid multiplexed target detection with common super-resolution techniques such as (d)STORM, STED, and SIM. We assay our approach using DNA origami nanostructures to quantitatively assess labeling, imaging, and washing efficiency. We furthermore demonstrate the applicability of our approach by imaging multiple protein targets in fixed cells.

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Language(s): eng - English
 Dates: 2017-03-222017-03-27
 Publication Status: Published in print
 Pages: 4
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000397346200052
DOI: 10.1002/anie.201611729
 Degree: -

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Title: Angewandte Chemie International Edition
  Other : Angew. Chem., Int. Ed.
  Other : Angew. Chem. Int. Ed.
  Other : Angewandte Chemie, International Edition
Source Genre: Journal
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Publ. Info: Weinheim : Wiley-VCH
Pages: - Volume / Issue: 56 (14) Sequence Number: - Start / End Page: 4052 - 4055 Identifier: ISSN: 1433-7851
CoNE: https://pure.mpg.de/cone/journals/resource/1433-7851