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  Fluorescent photoswitchable diarylethenes for biolabeling and single molecule localization microscopies with optical superresolution.

Roubinet, B., Weber, M., Shojaei, H., Bates, M., Bossi, M. L., Belov, V. N., et al. (2017). Fluorescent photoswitchable diarylethenes for biolabeling and single molecule localization microscopies with optical superresolution. Journal of the American Chemical Society, 139(19), 6611-6620. doi:10.1021/jacs.7b00274.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-3006-7 Version Permalink: http://hdl.handle.net/21.11116/0000-0001-4B1F-A
Genre: Journal Article

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 Creators:
Roubinet, B.1, Author              
Weber, M.1, Author              
Shojaei, H.1, Author              
Bates, M.1, Author              
Bossi, M. L.1, Author              
Belov, V. N.1, Author              
Irie, M., Author
Hell, S. W.1, Author              
Affiliations:
1Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society, ou_578627              

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 Abstract: A modular assembly of water soluble diarylethenes (DAEs), applicable as bio-markers for optical nanoscopy, is reported. Reversibly photoswitchable 1,2-bis(2-alkyl-6-phenyl-1-benzothiophene-1,1-dioxide-3-yl)perfluorocyclopentenes possessing a fluorescent "closed" form were decorated with one or two methoxy group(s) attached to the para-position(s) of phenyl ring(s) and two, four or eight carboxylic acid groups. Antibody conjugates of these DAEs feature low aggregation, efficient photoswitching in aqueous buffers, specific staining of cellular structures and photophysical properties (high emission efficiencies and low cycloreversion quantum yields) enabling their application in superresolution microscopy. Images of tubulin, vimentin and nuclear pore complexes are presented. The superresolution images can also be acquired by using solely 488 nm light without additional photoactivation with UV light. These DAEs exhibit reversible photoswitching without requiring any additives to the imaging media and open new paths towards the modular design of fluorescent dyes for bioimaging with optical superresolution.

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Language(s): eng - English
 Dates: 2017-04-242017-05-17
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1021/jacs.7b00274
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Title: Journal of the American Chemical Society
Source Genre: Journal
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Pages: - Volume / Issue: 139 (19) Sequence Number: - Start / End Page: 6611 - 6620 Identifier: -